Cadherin-18 Polyclonal antibody

Cadherin-18 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13091-1-AP

Synonyms

cadherin 18, type 2, Cadherin-14, Cadherin-18, CDH14, CDH14L, CDH18, EY CADHERIN

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Validation Data Gallery

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  WB analysis of COLO 320 using 13091-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13091-1-AP (Cadherin-18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13091-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13091-1-AP (Cadherin-18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13091-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13091-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 13091-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 13091-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, A549 cells
• Positive IHC detected in: human gliomas tissue, rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13091-1-AP targets Cadherin-18 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cadherin-18 fusion protein Ag3675
• Full Name: cadherin 18, type 2
• Calculated Molecular Weight: 790 aa, 88 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC031051
• Gene Symbol: Cadherin-18
• Gene ID (NCBI): 1016
• RRID: AB_10646435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	NPJ Regen Med; CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cells.; Authors - Julia Junghof
human	WB,IHC	Cell Physiol Biochem; A Novel Tumor-Suppressor, CDH18, Inhibits Glioma Cell Invasiveness Via UQCRC2 and Correlates with the Prognosis of Glioma Patients.; Authors - Ya-Hui Bai