Cadherin-20 Polyclonal antibody

Cadherin-20 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22049-1-AP

Synonyms

CDH20, CDH7L3, Cadherin 20

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Validation Data Gallery

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  WB analysis using 22049-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 22049-1-AP (Cadherin-20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22049-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22049-1-AP (Cadherin-20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22049-1-AP (Cadherin-20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, HeLa cells, mouse brain tissue
• Positive IHC detected in: human colon tissue, mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22049-1-AP targets Cadherin-20 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cadherin-20 fusion protein Ag16990
• Full Name: cadherin 20, type 2
• Calculated Molecular Weight: 801 aa, 89 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC101048
• Gene Symbol: CDH20
• Gene ID (NCBI): 28316
• RRID: AB_11125960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.