CDH26 Recombinant monoclonal antibody

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  WB analysis using 84143-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84143-1-RR (CDH26 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of U-2 OS using 84143-1-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug CDH26 Recombinant antibody (84143-1-RR, Clone:240934F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  FC experiment of HeLa using 84143-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDH26 Recombinant antibody (84143-1-RR, Clone:240934F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CDH26 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84143-1-RR

Clone No.240934F9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

240934F9, Cadherin-like protein 26, Cadherin-like protein VR20

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, HEK-293 cells, T-47D cells
• Positive FC (Intra) detected in: U-2 OS, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84143-1-RR targets CDH26 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cadherin-like 26
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: NM_021810
• Gene Symbol: CDH26
• Gene ID (NCBI): 60437
• RRID: AB_3671705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8IXH8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDH26, also named VR20, is a member of cadherins which are calcium-dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. CDH26 produces 4 isoforms through alternative splicing, with molecular weights of 13, 18, 92, and 95 kDa, respectively.