P-cadherin Monoclonal antibody

P-cadherin Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1G10F7

Cat no : 66295-1-Ig

Synonyms

Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin



Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66295-1-Ig targets P-cadherin in WB, IHC, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P-cadherin fusion protein Ag4758
Full Name cadherin 3, type 1, P-cadherin (placental)
Calculated molecular weight 829 aa, 91 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC041846
Gene symbol CDH3
Gene ID (NCBI) 1001
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

Protocols

Product Specific Protocols
WB protocol for P-cadherin antibody 66295-1-IgDownload protocol
IHC protocol for P-cadherin antibody 66295-1-IgDownload protocol
FC protocol for P-cadherin antibody 66295-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols