Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 13060-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13060-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13060-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13060-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 13060-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 13060-2-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2AP1 antibody (13060-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

CDK2AP1 Polyclonal antibody

CDK2AP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13060-2-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CDK2 associated protein 1, CDK2-associated protein 1, CDKAP1, Cyclin-dependent kinase 2-associated protein 1, Deleted in oral cancer 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells
Positive IHC detected in	human placenta tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

13060-2-AP targets CDK2AP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CDK2AP1 fusion protein Ag3736 Predict reactive species
Full Name	cyclin-dependent kinase 2 associated protein 1
Calculated Molecular Weight	115 aa, 13 kDa
Observed Molecular Weight	12 kDa
GenBank Accession Number	BC034717
Gene Symbol	CDK2AP1
Gene ID (NCBI)	8099
RRID	AB_10340136
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14519
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CDK2AP1 antibody 13060-2-AP	Download protocol
IHC protocol for CDK2AP1 antibody 13060-2-AP	Download protocol
IF protocol for CDK2AP1 antibody 13060-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncol Lett Cathepsin L interacts with CDK2-AP1 as a potential predictor of prognosis in patients with breast cancer. Authors - Zhan Wang View Article