CDK2AP1 Polyclonal antibody

CDK2AP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13060-2-AP

Synonyms

DOC 1, Deleted in oral cancer 1, Cyclin-dependent kinase 2-associated protein 1, CDKAP1, CDK2-associated protein 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 13060-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13060-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13060-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13060-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13060-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2AP1 antibody (13060-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human testis tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13060-2-AP targets CDK2AP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK2AP1 fusion protein Ag3736
• Full Name: cyclin-dependent kinase 2 associated protein 1
• Calculated Molecular Weight: 115 aa, 13 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC034717
• Gene Symbol: CDK2AP1
• Gene ID (NCBI): 8099
• RRID: AB_10340136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncol Lett; Cathepsin L interacts with CDK2-AP1 as a potential predictor of prognosis in patients with breast cancer.; Authors - Zhan Wang