CDK3 Polyclonal antibody

CDK3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 55103-1-AP

Synonyms

CDKN3, EC:2.7.11.22, Cyclin-dependent kinase 3, Cyclin dependent kinase 3, Cell division protein kinase 3

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Validation Data Gallery

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  WB analysis using 55103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55103-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55103-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55103-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 55103-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55103-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55103-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK3 antibody (55103-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 55103-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK3 Polyclonal antibody (55103-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: mouse brain tissue, MCF-7 cells, Jurkat cells, rat brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

55103-1-AP targets CDK3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cyclin-dependent kinase 3
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_001258
• Gene Symbol: CDK3
• Gene ID (NCBI): 1018
• RRID: AB_10804352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.

Publications

Species	Application	Title
human	IHC	Cancer Med; Identification of an EMT-related gene-based prognostic signature in osteosarcoma; Authors - Haoli Gong
mouse	WB	Protein J; Characterization of Protein Profiling and mRNA Expression of LLC Exosomes.; Authors - Wen Zhang
human	WB	Cell Death Discov; Up-regulation of SLC27A2 suppresses the proliferation and invasion of renal cancer by down-regulating CDK3-mediated EMT.; Authors - Ning Xu
human	WB	Cancer Med; The expression and role of SUZ12 in lung adenocarcinoma; Authors - Xingsheng Hu