Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, Jurkat cells, MCF-7 cells, rat brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55103-1-AP targets CDK3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cyclin-dependent kinase 3 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | NM_001258 |
| Gene Symbol | CDK3 |
| Gene ID (NCBI) | 1018 |
| RRID | AB_10804352 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00526 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CDK3 antibody 55103-1-AP | Download protocol |
| IF protocol for CDK3 antibody 55103-1-AP | Download protocol |
| IHC protocol for CDK3 antibody 55103-1-AP | Download protocol |
| WB protocol for CDK3 antibody 55103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDK3 Polyclonal antibody (Cat# 55103-1-AP) has 6 total citations. These appear in Cell Death Discovery, Cell Chemical Biology, RSC Medicinal Chemistry, Cancer Medicine (×2), and Protein Journal. The research fields span renal cancer and EMT, CDK2/CDK8 PROTAC degrader development for cancer therapy, lung adenocarcinoma, osteosarcoma prognostic signatures, and exosome protein profiling. Applications cited include WB and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Up-regulation of SLC27A2 suppresses the proliferation and invasion of renal cancer by down-regulating CDK3-mediated EMT. | ||
Cell Chem Biol CDK2 heterobifunctional degraders co-degrade CDK2 and cyclin E resulting in efficacy in CCNE1-amplified and overexpressed cancers | ||
RSC Med Chem Discovery of a potent dual-target CDK8 and cyclin C PROTAC degrader for the treatment of colorectal cancer. | ||
Cancer Med Identification of an EMT-related gene-based prognostic signature in osteosarcoma | ||
