CDK3 Polyclonal antibody

CDK3 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 55103-1-AP

Synonyms

CDK3, Cell division protein kinase 3, cyclin dependent kinase 3



Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells, Jurkat cells, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

55103-1-AP targets CDK3 in WB, IHC, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cyclin-dependent kinase 3
Calculated molecular weight 35 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberNM_001258
Gene symbol CDK3
Gene ID (NCBI) 1018
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.

Protocols

Product Specific Protocols
WB protocol for CDK3 antibody 55103-1-APDownload protocol
IHC protocol for CDK3 antibody 55103-1-APDownload protocol
FC protocol for CDK3 antibody 55103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Protein J

Characterization of Protein Profiling and mRNA Expression of LLC Exosomes.

Authors - Wen Zhang