CDK3 Polyclonal antibody

CDK3 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 55103-1-AP


CDK3, Cell division protein kinase 3, cyclin dependent kinase 3

Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells, Jurkat cells, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55103-1-AP targets CDK3 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cyclin-dependent kinase 3
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberNM_001258
Gene Symbol CDK3
Gene ID (NCBI) 1018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.


Product Specific Protocols
WB protocol for CDK3 antibody 55103-1-APDownload protocol
IHC protocol for CDK3 antibody 55103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Med

Identification of an EMT-related gene-based prognostic signature in osteosarcoma

Authors - Haoli Gong

Protein J

Characterization of Protein Profiling and mRNA Expression of LLC Exosomes.

Authors - Wen Zhang

Cell Death Discov

Up-regulation of SLC27A2 suppresses the proliferation and invasion of renal cancer by down-regulating CDK3-mediated EMT.

Authors - Ning Xu