CDK4 Recombinant monoclonal antibody, PBS Only

Name: CDK4 Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 83994-3-PBS-100UG

CDK4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 83994-3-PBS

Clone No. 241053F10

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

241053F10, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, Cyclin-dependent kinase 4

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBIPIF/ICC

WB analysis using 83994-3-RR (same clone as 83994-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83994-3-RR (CDK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 83994-3-RR (same clone as 83994-3-PBS)
WB result of CDK4 antibody (83994-3-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK4 transfected HeLa cells. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 83994-3-RR (same clone as 83994-3-PBS)
IP result of anti-CDK4 (IP:83994-3-RR, 4ug; Detection:83994-3-RR 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 83994-3-RR (same clone as 83994-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (83994-3-RR, Clone: 241053F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83994-3-PBS targets CDK4 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag20538 Product name: Recombinant human CDK4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-303 aa of BC010153 Sequence: MATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE Predict reactive species
Full Name	cyclin-dependent kinase 4
Calculated Molecular Weight	34 kDa
Observed Molecular Weight	30-34 kDa
GenBank Accession Number	BC010153
Gene Symbol	CDK4
Gene ID (NCBI)	1019
RRID	AB_3671570
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P11802
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1-to S-phase transition during the cell cycle, and its expression is primarily controlled at the transcriptional level(PMID:17253961). The CCND1-CDK4 axis is critical not only in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment, which are vital to sustain tumor outgrowth (PMID:21844184).