Various lysates were subjected to SDS PAGE followed by western blot with 84749-1-RR (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84749-1-RR (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 84749-1-RR
IP result of anti-CDK5 (IP:84749-1-RR, 4ug; Detection:84749-1-RR 1:8000) with mouse brain tissue lysate 2120 ug.
IP result of anti-CDK5 (IP:84749-1-RR, 4ug; Detection:84749-1-RR 1:8000) with mouse brain tissue lysate 2120 ug.
IHC staining of rat brain using 84749-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 84749-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 84749-1-RR
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (84749-1-RR, Clone: 241908F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
NIH/3T3 cells, RAW 264.7 cells, mouse brain tissue
Positive IP detected in
mouse brain tissue
Positive IHC detected in
rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
84749-1-RR targets CDK5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.
Various lysates were subjected to SDS PAGE followed by western blot with 84749-1-RR (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of rat brain using 84749-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 84749-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse brain using 84749-1-RR
IP result of anti-CDK5 (IP:84749-1-RR, 4ug; Detection:84749-1-RR 1:8000) with mouse brain tissue lysate 2120 ug.
IF/ICC Figures
IF Staining of SH-SY5Y using 84749-1-RR
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (84749-1-RR, Clone: 241908F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CDK5 Recombinant antibody
Catalog Number
84749-1-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:250-1:1000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
