CDK5RAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDK5RAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14740-1-AP

Synonyms

C20orf34, C42, CDK5RAP1, CDK5RAP1.3, CDK5RAP1.4, CGI 05, HSPC167

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Validation Data Gallery

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  WB analysis using 14740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14740-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14740-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 14740-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 14740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat brain using 14740-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat brain using 14740-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 14740-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14740-1-AP (CDK5RAP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse brain using 14740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14740-1-AP (CDK5RAP1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: C6 cells, human heart tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human pancreas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

14740-1-AP targets CDK5RAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK5RAP1 fusion protein Ag6409
• Full Name: CDK5 regulatory subunit associated protein 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC050706
• Gene Symbol: CDK5RAP1
• Gene ID (NCBI): 51654
• RRID: AB_2076843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; METTL17 is an Fe-S cluster checkpoint for mitochondrial translation; Authors - Tslil Ast ; KD Validated