CDK7 Polyclonal antibody

CDK7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 27027-1-AP

Synonyms

CDKN7, CDK-activating kinase 1, CDK activating kinase, CAK1, CAK

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Validation Data Gallery

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  WB analysis of HepG2 using 27027-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27027-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27027-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 27027-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 27027-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27027-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 27027-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK7 antibody (27027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, mouse embryo tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

27027-1-AP targets CDK7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK7 fusion protein Ag25726
• Full Name: cyclin-dependent kinase 7
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC005298
• Gene Symbol: CDK7
• Gene ID (NCBI): 1022
• RRID: AB_2880728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	ACS Nano; Nanomaterial-Facilitated Cyclin-Dependent Kinase 7 Inhibition Suppresses Gallbladder Cancer Progression via Targeting Transcriptional Addiction.; Authors - Chen-Song Huang
mouse	WB	Genes Dev; TRF1 uses a noncanonical function of TFIIH to promote telomere replication; Authors - Zhe Yang
human	WB	Front Oncol; Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach; Authors - Xiaobin Fei
human	WB	Cell Signal; TAGLN2 targeted control of ARPC5-mediated activation of the MEK/ERK signaling pathway influences the proliferation, invasion, and metastasis of pancreatic cancer cells; Authors - Yongjia Gao
human,mouse	WB	J Immunother Cancer; Adenovirus vaccine targeting kinases induces potent antitumor immunity in solid tumors; Authors - Fei Zhu