CDKL5 Polyclonal antibody

CDKL5 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12973-1-AP

Synonyms

CDKL5, cyclin dependent kinase like 5, ISSX, STK9

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Validation Data Gallery

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  WB analysis using 12973-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12973-1-AP (CDKL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12973-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12973-1-AP (CDKL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12973-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12973-1-AP (CDKL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12973-1-AP

  IP result of anti-CDKL5 (IP:12973-1-AP, 4ug; Detection:12973-1-AP 1:300) with mouse brain tissue lysate 3200 ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, PC-12 cells, SH-SY5Y cells, HeLa cell
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

12973-1-AP targets CDKL5 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDKL5 fusion protein Ag3531
• Full Name: cyclin-dependent kinase-like 5
• Calculated Molecular Weight: 1030 aa, 114 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC036091
• Gene Symbol: CDKL5
• Gene ID (NCBI): 6792
• RRID: AB_2877899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin dependent kinase like 5 (CDKL5) may regulate ciliogenesis and neuronal morphogenesis. It has been reported that CDKL5 is associated with epileptic seizures, severe intellectual disability, and autistic features. The CDKL5 protein is a kinase that regulates key phosphorylation events vital to the development of the complex neuronal network of the brain.

Publications

Species	Application	Title
mouse	WB,IF	Neurosci Res; Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder.; Authors - Alessia Di Nardo
human	WB	Cell Rep; A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder; Authors - Hanzhe Kuang