Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, TF-1 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00038 targets CDKN2A/P16-INK4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | cyclin-dependent kinase inhibitor 2A |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16-18 kDa |
| GenBank Accession Number | BC021998 |
| Gene Symbol | CDKN2A |
| Gene ID (NCBI) | 1029 |
| RRID | AB_3741448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | P42771 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins proteins were reported. Two of them named p16-INK4 and p14 are sharing 50% identity. The third one -p14(ARF) is entirely unrelated. P16 plays an essential role in regulating the cell cycle, and mutations in p16 increase the risk of developing various cancers, including melanoma.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CDKN2A/P16-INK4A antibody RMX00038 | Download protocol |
| IHC protocol for CDKN2A/P16-INK4A antibody RMX00038 | Download protocol |
| WB protocol for CDKN2A/P16-INK4A antibody RMX00038 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RMX00038 is the Multi-rAb® CDKN2A/P16-INK4A Multi-Recombinant antibody, with a total of 2 citations. The citations appear in Advanced Healthcare Materials (IF 11) and Journal of Hazardous Materials. The research fields span cartilage senescence and cellular remodeling via urokinase receptor clustering, as well as nanoplastic biodistribution and ROS-mediated intestinal senescence.
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Remodeling Cellular Uptake via Inducing Clustering of Urokinase Receptors in Hydrogel Microspheres for Alleviating Cartilage Senescence. | ||
J Hazard Mater Dietary patterns reshape organ-specific biodistribution and toxicity upon exposure to polystyrene nanoplastics: Role of ROS-mediated intestinal senescence. |
