CDKN2A/P16-INK4A Polyclonal antibody

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• CDKN2A/P16-INK4A Polyclonal antibody (28416-1-AP)

CDKN2A/P16-INK4A Polyclonal Antibody for WB, ELISA

Cat No. 28416-1-AP Copied!

Publications (36)

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Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Cdkn2a/p16INK4a, p16 INK4, p16INK4a, Cdkn2a, Cyclin-dependent kinase inhibitor 2A

• 

  WB analysis using 28416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (CDKN2A/P16-INK4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 28416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (p16INK4a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells, mouse uterus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28416-1-AP targets CDKN2A/P16-INK4A in WB, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28927

  Product name: Recombinant mouse Cdkn2a protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-168 aa of NM_001040654

  Sequence: MESAADRLARAAAQGRVHDVRALLEAGVSPNAPNSFGRTPIQVMMMGNVHVAALLLNYGADSNCEDPTTFSRPVHDAAREGFLDTLVVLHGSGARLDVRDAWGRLPLDLAQERGHQDIVRYLRSAGCSLCSAGWSLCTAGNVAQTDGHSFSSSTPRALELRGQSQEQS

• Full Name: cyclin-dependent kinase inhibitor 2A
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18-23 kDa
• GenBank Accession Number: NM_001040654
• Gene Symbol: Cdkn2a
• Gene ID (NCBI): 12578
• RRID: AB_3086048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51480
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

p16INK4a is a pivotal protein encoded by the CDKN2A gene on human chromosome 9p21. It functions as a tumor suppressor and is one of the most important regulators of the cell cycle. It acts as a cyclin-dependent kinase (CDK) inhibitor, specifically binding to CDK4 and CDK6 and blocking their association with cyclin D. Beyond cell-cycle control, p16INK4a is a key mediator of cellular senescence-an irreversible growth-arrest state that limits the expansion of damaged or oncogene-expressing cells. Its expression rises with age and in response to stresses such as DNA damage or oncogenic signaling, making it a biomarker of both biological aging and tumorigenic stress. In human cancers, the p16INK4a-RB pathway is frequently disabled through CDKN2A deletion, mutation, or promoter hyper-methylation, underscoring its importance in tumor suppression.

Publications

What published studies show

The CDKN2A/P16-INK4A polyclonal antibody (Cat# 28416-1-AP) has 36 citations published in journals including Nature Aging, Nature Communications, Biomaterials, Advanced Materials, Autophagy, Cell Reports, Aging Cell, Journal of Controlled Release, Free Radical Biology and Medicine, and others. Research fields span cellular senescence and aging, intervertebral disc degeneration, osteoarthritis, liver fibrosis, Alzheimer's disease, pulmonary fibrosis, cardiovascular aging, osteoporosis, rheumatoid arthritis, and prostate cancer.

Automatically generated from the 36 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat,mouse	WB,IF	Adv Mater; Ionizable Coenzyme-Engineered Lipid/Fiber Microplexes Boost Ribosomal Translation to Improve mRNA Therapy for Degenerative Diseases.; Authors - Shifeng Ling
mouse,human	WB	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
		Autophagy; Oxidative stress-preconditioned exosomes target BMF to restore mitophagy for alleviating intervertebral disc degeneration.; Authors - Yun Teng
		Nat Commun; Targeting NAT10 alleviates colonic senescence and elderly-onset colitis by disrupting N4-acetylation of DYRK1A; Authors - Jingyu Chen
rat	IF	Adv Sci (Weinh); Inhibiting cGAS-STING to Preserve Mitochondrial-Nuclear Communication and Stemness in Young Tendon Stem Cells: A Hydrogel Strategy against Age-Related Tendinopathy.; Authors - Zhuo Zhang
rat	IHC,IF	Biomaterials; Ultrasound-responsive piezoelectric hydrogel attenuates oxidative stress-induced nucleus pulposus senescence via AMPK-FOXO1a-mediated mitophagy for intervertebral disc regeneration.; Authors - Wenbo Wu