CDKN2AIP Polyclonal antibody

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• CDKN2AIP Polyclonal antibody (16615-1-AP)

CDKN2AIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16615-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CARF, CDKN2A-interacting protein

• 

  WB analysis using 16615-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 16615-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16615-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 16615-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 16615-1-AP

  IP result of anti-CDKN2AIP (IP:16615-1-AP, 4ug; Detection:16615-1-AP 1:500) with HeLa cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 16615-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDKN2AIP antibody (16615-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, Jurkat cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue, human stomach cancer tissue, mouse testis tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16615-1-AP targets CDKN2AIP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9907

  Product name: Recombinant human CDKN2AIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 230-579 aa of BC022270

  Sequence: ASEAEAPDKHGSSFVSLLKSSVNSHMTQSTDSRQQSGSPKKSALEGSSASASQSSSEIEVPLLGSSGSSEVELPLLSSKPSSETASSGLTSKTSSEASVSSSVAKNSSSSGTSLLTPKSSSSTNTSLLTSKSTSQVAASLLASKSSSQTSGSLVSKSTSLASVSQLASKSSSQTSTSQLPSKSTSQSSESSVKFSCKLTNEDVKQKQPFFNRLYKTVAWKLVAVGGFSPNVNHGELLNAAIEALKATLDVFFVPLKELADLPQNKSSQESIVCELRCKSVYLGTGCGKSKENAKAVASREALKLFLKKKVVVKICKRKYRGSEIEDLVLLDEESRPVNLPPALKHPQELL

• Full Name: CDKN2A interacting protein
• Calculated Molecular Weight: 579 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC022270
• Gene Symbol: CDKN2AIP
• Gene ID (NCBI): 55602
• RRID: AB_2244775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NXV6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CDKN2AIP Polyclonal antibody (Cat# 16615-1-AP) has 6 citations published in Cell Biosciences, Journal of Diabetes, Acta Biochimica et Biophysica Sinica, International Journal of Cardiology, Clinical, Cosmetic and Investigational Dermatology, and Advanced Science. Research fields span reproductive biology (spermatogenesis), diabetes/β-cell senescence, cardiology (cardiomyopathy), dermatology (nevus sebaceous), and oncology (meningioma radioresistance).

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC,IF	Cell Biosci; CDKN2AIP is critical for spermiogenesis and germ cell development; Authors - Yuming Cao
mouse	WB,CoIP	J Diabetes; Taurine Alleviates Pancreatic β-Cell Senescence by Inhibition of p53 Pathway; Authors - Baomin Wang
mouse	IF	Acta Biochim Biophys Sin (Shanghai); CARF regulates the alternative splicing and piwi/piRNA complexes during mouse spermatogenesis through PABPC1; Authors - Yuming Cao
mouse	WB,IF	Int J Cardiol; Reduced myocardial CARF and the underlying implications in left ventricular noncompaction cardiomyopathy; Authors - Liu Hui ; KO Validated
human	WB	Clin Cosmet Investig Dermatol; Genome Wide Differential Expression Profiles in Nevus Sebaceous Uncovered Low Expression of CDKN2AIP and Construction of a ceRNA Network.; Authors - Xianhong Yang
human	WB	Adv Sci (Weinh); Lactylation-Driven PROS1-TYRO3-CARF Signaling Promotes Therapy-Induced Senescence Escape and Radioresistance in Meningioma.; Authors - Zhuohang Wang