CDO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDO1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 12589-1-AP

Synonyms

CDO, CDO I, CDO1, Cysteine dioxygenase type 1, cysteine dioxygenase, type I

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Validation Data Gallery

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  WB analysis of mouse liver using 12589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:3000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse liver using 12589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 12589-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 12589-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse liver using 12589-1-AP

  IP result of anti-CDO1 (IP:12589-1-AP, 3ug; Detection:12589-1-AP 1:600) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human placenta tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 7 publications below

Product Information

12589-1-AP targets CDO1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDO1 fusion protein Ag3294
• Full Name: cysteine dioxygenase, type I
• Calculated Molecular Weight: 200 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC024241
• Gene Symbol: CDO1
• Gene ID (NCBI): 1036
• RRID: AB_10638145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDO1(cysteine dioxygenase type 1) is also named as CDO and belongs to the cysteine dioxygenase family. It is an enzyme that adds molecular oxygen to the sulfur of cysteine, converting the thiol to a sulfinic acid known as cysteinesulfinic acid (3-sulfinoalanine) and CDO1 is one of the most highly regulated metabolic enzymes responding to diet(PMID:19011731). The expression of CDO can significantly decrease the level of intracellular cysteine and this reduction is also associated with a decrease in total glutathione levels(PMID:17327371). Cysteine dioxygenase (CDO) plays a critical role in the regulation of cellular cysteine concentration andmultiple forms of CDO (23 kDa, 25 kDa, and 68 kDa) have been claimed based upon separation and detection using SDS-PAGE/western blotting(PMID: 14752623).

Publications

Species	Application	Title
human	WB,IHC	Cell Death Discov; Cysteine dioxygenase 1 attenuates the proliferation via inducing oxidative stress and integrated stress response in gastric cancer cells; Authors - Gang Ma
human	IHC	Int J Biol Sci; Ferroptosis-associated molecular classification characterized by distinct tumor microenvironment profiles in colorectal cancer.; Authors - Wenqin Luo
mouse	WB	J Nutr Biochem; PMID: 24917045; Authors - Kerstin E Geillinger
rat	WB	J Ethnopharmacol; Integration of metabolomics and transcriptomics to reveal anti-chronic myocardial ischemia mechanism of Gualou Xiebai decoction.; Authors - Fengyun Zhang
human	IHC	Ann Surg Oncol; Promoter DNA Hypermethylation of the Cysteine Dioxygenase 1 (CDO1) Gene in Intraductal Papillary Mucinous Neoplasm (IPMN).; Authors - Yoshiki Fujiyama
mouse	WB	Cell Mol Neurobiol; Taurine Alleviates Chronic Social Defeat Stress-Induced Depression by Protecting Cortical Neurons from Dendritic Spine Loss.; Authors - Yuanyuan Zhu