CDSN Polyclonal antibody

• Featured Product
• KD/KO Validated

CDSN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13184-1-AP

Synonyms

S protein, HTSS, D6S586E, corneodesmosin

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Validation Data Gallery

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  WB analysis using 13184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13184-1-AP (CDSN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 13184-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13184-1-AP (CDSN antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 13184-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13184-1-AP (CDSN antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, NIH/3T3 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

13184-1-AP targets CDSN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDSN fusion protein Ag3854
• Full Name: corneodesmosin
• Calculated Molecular Weight: 528 aa, 52 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC031993
• Gene Symbol: CDSN
• Gene ID (NCBI): 1041
• RRID: AB_11183052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDSN, also named as S protein, is important for the epidermal barrier integrity. CDSN is a unique extracellular 52- to 56-kDa glycoprotein found in corneodesmosomes. Pathogenic mutations in CDSN have been linked in humans to genodermatoses affecting both the hair follicle and the epidermis. Inflammatory peeling skin syndrome is caused a novel mutation in CDSN.

Publications

Species	Application	Title
mouse	WB	J Invest Dermatol; Sphingosine 1-Phosphate Receptor 2 Is Central to Maintaining Epidermal Barrier Homeostasis.; Authors - Satomi Igawa
human	IF	Exp Dermatol; Acral peeling in Nagashima type palmo-plantar keratosis patients reveals the role of serine protease inhibitor B 7 in keratinocyte adhesion.; Authors - Eran Cohen-Barak
human	IHC	Clin Cosmet Investig Dermatol; Repeated short climatic change affects the epidermal differentiation program and leads to matrix remodeling in a human organotypic skin model.; Authors - Laetitia-Barbollat Boutrand
human	IHC	Arch Dermatol Res; Inflammatory peeling skin syndrome caused a novel mutation in CDSN.; Authors - Telem Dana Fuchs DF
human	WB,IHC	Heliyon; Diagnostic, prognostic, and immunological roles of CDSN in ovarian cancer; Authors - Huan Zeng ; KD Validated
mouse	IF	Nat Neurosci; Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice; Authors - Timothy M O'Shea