Tested Applications
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14382-1-AP targets CDT1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5810 Product name: Recombinant human CDT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 195-546 aa of BC000137 Sequence: EMFRSMDTIVGMLHNRSETPTFAKVQRGVQDMMRRRFEERNVGQIKTVYPASYRFRQERSVPTFKDGTRRSDYQLTIEPLLEQEADGAAPQLTASRLLQRRQIFSQKLVEHVKEHHKAFLASLSPAMVVPEDQLTRWHPRFNVDEVPDIEPAALPQPPATEKLTTAQEVLARARNLISPRMEKALSQLALRSAAPSSPGSPRPALPATPPATPPAASPSALKGVSQDLLERIRAKEAQKQLAQMTRCPEQEQRLQRLERLPELARVLRSVFVSERKPALSMEVACARMVGSCCTIMSPGEMEKHLLLLSELLPDWLSLHRIRTDTYVKLDKAADLAHITARLAHQTRAEEGL Predict reactive species |
| Full Name | chromatin licensing and DNA replication factor 1 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC000137 |
| Gene Symbol | CDT1 |
| Gene ID (NCBI) | 81620 |
| RRID | AB_2076871 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H211 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDT1 antibody 14382-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-CDT1 antibody (Cat# 14382-1-AP) has 15 citations across journals including Nature, Nature Communications, Cell Reports Medicine, Science Advances, Journal of Medicinal Chemistry, Scientific Reports, ACS Pharmacology & Translational Science, Frontiers in Oncology, PeerJ, FEBS Open Bio, Journal of Clinical Biochemistry and Nutrition, Genes & Cells, and Oncotarget. Research fields span cancer biology (hepatocellular carcinoma, glioma, lung adenocarcinoma, AML, biliary tract cancer), cell cycle regulation, DNA replication, and biomarker discovery.
| Species | Application | Title |
|---|---|---|
Nature Skp2 targeting suppresses tumorigenesis by Arf-p53-independent cellular senescence. | ||
Nat Commun IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion. | ||
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
J Med Chem Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction. | ||
Sci Rep The proliferation of atypical hepatocytes and CDT1 expression in noncancerous tissue are associated with the postoperative recurrence of hepatocellular carcinoma |
