CDX2 Polyclonal antibody, PBS Only

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• CDX2 Polyclonal antibody, PBS Only (55286-1-PBS)

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  WB analysis of COLO 320 using 55286-1-AP (same clone as 55286-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55286-1-AP (CDX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55286-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 55286-1-AP (same clone as 55286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55286-1-AP (CDX2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55286-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55286-1-AP (same clone as 55286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 55286-1-AP (CDX2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55286-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 55286-1-AP (same clone as 55286-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (55286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 55286-1-PBS in a different storage buffer formulation.

CDX2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 55286-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

55286-1-PBS targets CDX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: caudal type homeobox 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: NM_001265
• Gene Symbol: CDX2
• Gene ID (NCBI): 1045
• RRID: AB_3670245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99626
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.