Filter: AllIHCAffinity

IHC staining of human colon using 82663-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82663-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 82663-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82663-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 82663-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82663-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 82663-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82663-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 82663-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82663-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 82663-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82663-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Affinity of 82663-1-RR
Biolayer interferometry (BLl) kinetic assays of 82663-1-RR against Human CDX2 were performed. The affinity constant is 10.8 nM.

CDX2 Recombinant monoclonal antibody

Name: CDX2 Recombinant monoclonal antibody
Brand: Proteintech
SKU: 82663-1-RR

CDX2 Uni-rAb^® Recombinant Antibody for IHC, ELISA

Cat No. 82663-1-RR

Clone No.1E4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

Caudal type homeobox 2, Caudal type homeobox protein 2, Caudal-type homeobox protein 2, CDX 3, CDX-2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in

human colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82663-1-RR targets CDX2 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag17310 Product name: Recombinant human CDX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC014461 Sequence: MYVSYLLDKDVSMYPSSVRHSGGLNLAPQNFVSPPQYPDYGGYHVAAAAAAAANLDSAQSPGPSWPAAYGAPLREDWNGYAPGGAAAAANAVAHGLNGGSPAAAMGYSSPADYHPHHHPHHHPHHPAAAPSCASGLLQTLNPGPPGPAATAAAEQLSPGGQRRNLCEWMRKPAQQSLGSQ Predict reactive species
Full Name	caudal type homeobox 2
Calculated Molecular Weight	313 aa, 34 kDa
GenBank Accession Number	BC014461
Gene Symbol	CDX2
Gene ID (NCBI)	1045
RRID	AB_3743411
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q99626
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.