Tested Applications
| Positive WB detected in | MCF-7 cells, human saliva |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83653-5-RR targets CEA/CD66e in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Fusion Protein Predict reactive species |
| Full Name | carcinoembryonic antigen-related cell adhesion molecule 5 |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_004363 |
| Gene Symbol | CEA |
| Gene ID (NCBI) | 1048 |
| RRID | AB_3671261 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P06731-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis. This antibody is raised against 35-685 aa of human CEA/CD66e and can cross-react with CEACAM1/CD66a and CEACAM3/CD66d.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEA/CD66e antibody 83653-5-RR | Download protocol |
| IHC protocol for CEA/CD66e antibody 83653-5-RR | Download protocol |
| WB protocol for CEA/CD66e antibody 83653-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One user (Jan Čapek, January 2025) gave this recombinant CEA/CD66e antibody a 5-star rating after using it at 1:250 dilution for immunofluorescence on BxPC-3 cells. The reviewer reported successful detection of CEA when paired with a TRITC-conjugated Goat Anti-Rabbit IgG(H+L) secondary antibody, confirming reliable performance in their IF workflow.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jan (Verified Customer) (01-06-2025) | Immunofluorescence analysis of CEA in BxPC-3 cells. Cells were incubated with CEA/CD66e Recombinant antibody (83653-5-RR) and TRITC-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2).
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