Mouse CEACAM1/CD66a Recombinant antibody, PBS Only (Capture/Detector)

• 

  WB analysis using 84409-4-RR (same clone as 84409-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84409-4-RR (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84409-4-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01297-2

  Sandwich ELISA standard curve of MP01297-2, Mouse CEACAM1/CD66a Recombinant Matched Antibody Pair - PBS only. 84409-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1423. 84409-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

• 

  Sample test of MP01297-2

  The mean CEACAM1/CD66a concentration was determined to be 67.98 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load and 10.51 ng/mL in mouse small intestine tissue extract based on a 1.6 mg/mL extract load.

• 

  IHC staining of mouse liver using 84409-4-RR (same clone as 84409-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84409-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84409-4-RR (same clone as 84409-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84409-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 84409-4-RR (same clone as 84409-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84409-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 84409-4-RR (same clone as 84409-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84409-4-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 84409-4-RR (same clone as 84409-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CEACAM1/CD66a antibody (84409-4-RR, Clone: 241856H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84409-4-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 84409-4-RR (same clone as 84409-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using CEACAM1/CD66a antibody (84409-4-RR, Clone: 241856H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84409-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01297-2

  Cytometric bead array standard curve of MP01297-2, MOUSE CEACAM1/CD66a Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84409-4-PBS. Detection antibody: 84409-3-PBS. Standard: Eg1423. Range: 0.313-40 ng/mL.

• 

  Cytometric bead array standard curve of MP01297-3

  Cytometric bead array standard curve of MP01297-3, MOUSE CEACAM1/CD66a Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84409-2-PBS. Detection antibody: 84409-4-PBS. Standard: Eg1423. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 84409-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84409-4-RR against Mouse CEACAM1/CD66a were performed. The affinity constant is below 1 pM.

CEACAM1/CD66a Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84409-4-PBS

Clone No.241856H5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241856H5, Bgp, Bgp1, BGP-1, Biliary glycoprotein 1

---

Product Information

84409-4-PBS targets CEACAM1/CD66a as part of a matched antibody pair:

MP01297-2: 84409-4-PBS capture and 84409-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01297-3: 84409-2-PBS capture and 84409-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CEACAM1/CD66a fusion protein Eg1423
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 120 kDa
• Gene Symbol: Ceacam1
• Gene ID (NCBI): 26365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P31809-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).