Tested Applications
Positive WB detected in | HeLa cells, mouse liver tissue |
Positive IF/ICC detected in | Hela cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
17209-1-AP targets CEACAM21 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CEACAM21 fusion protein Ag10992 Predict reactive species |
Full Name | carcinoembryonic antigen-related cell adhesion molecule 21 |
Calculated Molecular Weight | 191aa,22 kDa; 293aa,32 kDa |
Observed Molecular Weight | 32 kDa, 22 kDa |
GenBank Accession Number | BC012001 |
Gene Symbol | CEACAM21 |
Gene ID (NCBI) | 90273 |
RRID | AB_2077481 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q3KPI0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Carcinoembryonic antigen-related cell adhesion molecule 21 (CEACAM21) is a member of the CEACAM family that has diverse functions in cell adhesion, in intracellular and intercellular signaling, and during complex biological processes such as cancer progression, inflammation, angiogenesis, and metastasis (PMID: 23903773; 32488569). CEACAM21 acts as a heterophilic ligand for TIM-3, leading to the dissociation of Bat3 and increasing proximal TCR signaling and proapoptotic protein expression in late effector T cells, thereby mediating T cell suppression (PMID: 38490257).
Protocols
Product Specific Protocols | |
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WB protocol for CEACAM21 antibody 17209-1-AP | Download protocol |
IF protocol for CEACAM21 antibody 17209-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |