CEACAM21 Polyclonal antibody

CEACAM21 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17209-1-AP

Synonyms

CEACAM21, CEACAM3, R29124_1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 17209-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17209-1-AP (CEACAM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17209-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17209-1-AP (CEACAM21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 17209-1-AP

  Immunofluorescent analysis of Hela cells, using CEACAM21 antibody 17209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17209-1-AP targets CEACAM21 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEACAM21 fusion protein Ag10992
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 21
• Calculated Molecular Weight: 191aa,22 kDa; 293aa,32 kDa
• Observed Molecular Weight: 32 kDa, 22 kDa
• GenBank Accession Number: BC012001
• Gene Symbol: CEACAM21
• Gene ID (NCBI): 90273
• RRID: AB_2077481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.