CEACAM21 Polyclonal antibody

CEACAM21 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17209-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Carcinoembryonic antigen-related cell adhesion molecule 21, UNQ3098/PRO10075

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17209-1-AP targets CEACAM21 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEACAM21 fusion protein Ag10992 Predict reactive species
Full Name carcinoembryonic antigen-related cell adhesion molecule 21
Calculated Molecular Weight191aa,22 kDa; 293aa,32 kDa
Observed Molecular Weight 32 kDa, 22 kDa
GenBank Accession NumberBC012001
Gene Symbol CEACAM21
Gene ID (NCBI) 90273
RRIDAB_2077481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KPI0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carcinoembryonic antigen-related cell adhesion molecule 21 (CEACAM21) is a member of the CEACAM family that has diverse functions in cell adhesion, in intracellular and intercellular signaling, and during complex biological processes such as cancer progression, inflammation, angiogenesis, and metastasis (PMID: 23903773; 32488569). CEACAM21 acts as a heterophilic ligand for TIM-3, leading to the dissociation of Bat3 and increasing proximal TCR signaling and proapoptotic protein expression in late effector T cells, thereby mediating T cell suppression (PMID: 38490257).

Protocols

Product Specific Protocols
WB protocol for CEACAM21 antibody 17209-1-APDownload protocol
IF protocol for CEACAM21 antibody 17209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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