CEACAM21 Polyclonal antibody

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  WB analysis of HeLa using 17209-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17209-1-AP (CEACAM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17209-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17209-1-AP (CEACAM21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17209-1-AP

  Immunofluorescent analysis of Hela cells, using CEACAM21 antibody 17209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CEACAM21 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17209-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Carcinoembryonic antigen-related cell adhesion molecule 21, UNQ3098/PRO10075

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17209-1-AP targets CEACAM21 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEACAM21 fusion protein Ag10992
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 21
• Calculated Molecular Weight: 191aa,22 kDa; 293aa,32 kDa
• Observed Molecular Weight: 32 kDa, 22 kDa
• GenBank Accession Number: BC012001
• Gene Symbol: CEACAM21
• Gene ID (NCBI): 90273
• RRID: AB_2077481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3KPI0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carcinoembryonic antigen-related cell adhesion molecule 21 (CEACAM21) is a member of the CEACAM family that has diverse functions in cell adhesion, in intracellular and intercellular signaling, and during complex biological processes such as cancer progression, inflammation, angiogenesis, and metastasis (PMID: 23903773; 32488569). CEACAM21 acts as a heterophilic ligand for TIM-3, leading to the dissociation of Bat3 and increasing proximal TCR signaling and proapoptotic protein expression in late effector T cells, thereby mediating T cell suppression (PMID: 38490257).