CELSR2 Polyclonal antibody

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  IHC staining of mouse brain using 19940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19940-1-AP (CELSR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 19940-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CELSR2 antibody (19940-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CELSR2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 19940-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

EGF-like protein 2, EGFL2, EGF like protein 2, CDHF10, Cadherin family member 10

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19940-1-AP targets CELSR2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
• Calculated Molecular Weight: 318 kDa
• GenBank Accession Number: NM_001408
• Gene Symbol: CELSR2
• Gene ID (NCBI): 1952
• RRID: AB_3669318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HCU4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CELSR2, also named CDHF10, EGFL2, KIAA0279, and MEGF3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is a receptor that may have an important role in cell/cell signaling during nervous system formation.