CELSR3 Polyclonal antibody

• 

  IHC staining of mouse cerebellum using 28835-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28835-1-AP (CELSR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 28835-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CELSR3 antibody (28835-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CELSR3 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 28835-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IHC, IF-P, ELISA

Synonyms

Cadherin family member 11, CDHF11, CELSR3, EGF like protein 1, EGFL1, Flamingo homolog 1, FMI1, HFMI1, KIAA0812, MEGF2, RESDA1

---

Tested Applications

• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28835-1-AP targets CELSR3 in IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28746

  Product name: Recombinant human CELSR3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2285-2540 aa of NM_001407

  Sequence: MPGGSPGSAGLVRHLEEYAATLARNMELTYLNPMGLVTPNIMLSIDRMEHPSSPRGARRYPRYHSNLFRGQDAWDPHTHVLLPSQSPRPSPSEVLPTSSSIENSTTSSVVPPPAPPEPEPGISIIILLVYRTLGGLLPAQFQAERRGARLPQNPVMNSPVVSVAVFHGRNFLRGILESPISLEFRLLQTANRSKAICVQWDPPGLAEQHGVWTARDCELVHRNGSHARCRCSRTGTFGVLMDASPRERLEGDLELLA

• Full Name: cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila)
• Calculated Molecular Weight: 358 kDa
• GenBank Accession Number: NM_001407
• Gene Symbol: CELSR3
• Gene ID (NCBI): 1951
• RRID: AB_3086089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYQ7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Atypical cadherin Celsr3 plays critical roles during brain development, in collaboration with Fzd3. Celsr3 is required for postnatal cerebellar Purkinje cell maturation and synaptic plasticity, which has potential implications in neural disorders implicating the cerebellum.