CENPK Polyclonal antibody

• 

  WB analysis of HeLa using 26208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26208-1-AP (CENPK Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26208-1-AP

  IP result of anti-CENPK (IP:26208-1-AP, 4ug; Detection:26208-1-AP 1:600) with HeLa cells lysate 4000ug.

• Featured Product
• KD/KO Validated

CENPK Polyclonal Antibody for WB, IP, ELISA

Cat No. 26208-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

AF5alpha, CENP K, CENPK, centromere protein K, FKSG14, ICEN37, P33, Protein AF 5alpha, Solt

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

26208-1-AP targets CENPK in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23823

  Product name: Recombinant human CENPK protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-269 aa of BC005400

  Sequence: MNQEDLDPDSTTDVGDVTNTEEELIRECEEMWKDMEECQNKLSLIGTETLTDSNAQLSLLIMQVKCLTAELSQWQKKTPETIPLTEDVLITLGKEEFQKLRQDLEMVLSTKESKNEKLKEDLEREQRWLDEQQQIMESLNVLHSELKNKVETFSESRIFNELKTKMLNIKEYKEKLLSTLGEFLEDHFPLPDRSVKKKKKNIQESSVNLITLHEMLEILINRLFDVPHDPYVKISDSFWPPYVELLLRNGIALRHPEDPTRIRLEAFHQ

• Full Name: centromere protein K
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC005400
• Gene Symbol: CENPK
• Gene ID (NCBI): 64105
• RRID: AB_2880427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BS16
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Transl Oncol; m6A demethylase ALKBH5 maintains stemness of intrahepatic cholangiocarcinoma by sustaining BUB1B expression and cell proliferation; Authors - Yuan Gao
human	WB,IHC	Cancer Cell Int; Bioinformatics analysis combined with experiments predicts CENPK as a potential prognostic factor for lung adenocarcinoma.; Authors - Jiayu Ma ; KD Validated
human	WB	Toxicol Appl Pharmacol; The inhibition of centromere protein K causes anticancer effects in breast carcinoma via effects on the FAK/PI3K/AKT/mTOR pathway; Authors - Jiao Yu ; KD Validated
human	WB	Bioengineered; Overexpression of centromere protein K (CENPK) gene in Differentiated Thyroid Carcinoma promote cell Proliferation and Migration; Authors - Qizhi Li ; KD Validated
human	WB	Toxicol Appl Pharmacol; Centromere protein K enhances the activation of YAP1/TAZ signal cascade to drive the progression of clear cell renal cell carcinoma; Authors - Ning Nan ; KD Validated
human	WB	Onco Targets Ther; Downregulation of CENPK suppresses hepatocellular carcinoma malignant progression through regulating YAP1.; Authors - Jianlin Wang