CENPM Polyclonal antibody, PBS Only

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• CENPM Polyclonal antibody, PBS Only (19840-1-PBS)

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  WB analysis using 19840-1-AP (same clone as 19840-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19840-1-AP (CENPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19840-1-AP (same clone as 19840-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CENPM antibody (19840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 19840-1-AP (same clone as 19840-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CENPM antibody (19840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

CENPM Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 19840-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

19840-1-PBS targets CENPM in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13843

  Product name: Recombinant human CENPM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-180 aa of BC000705

  Sequence: MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL

• Full Name: centromere protein M
• Calculated Molecular Weight: 180 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC000705
• Gene Symbol: CENPM
• Gene ID (NCBI): 79019
• RRID: AB_3661902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NSP4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Centromere protein M (CENPM), also referred as proliferation associated nuclear element 1 (PANE1), is one of the components of a complex that plays a central role in kinetochore protein assembly, mitotic progression and chromosome segregation. The expression of CENPM and centromere assembly should be controlled closely during the cell cycle, and mistakes in this regulation may result in aneuploidy (PMID: 32705259). CENPM has been reported as a novel biomarker of hepatocellular carcinoma, melanoma and bladder cancer (PMID: 31703591).