CENPO Polyclonal antibody, PBS Only

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• CENPO Polyclonal antibody, PBS Only (20611-1-PBS)

• 

  WB analysis of mouse brain using 20611-1-AP (same clone as 20611-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 20611-1-AP (same clone as 20611-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 20611-1-AP (same clone as 20611-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20611-1-AP (same clone as 20611-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20611-1-AP (same clone as 20611-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 20611-1-AP (same clone as 20611-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

CENPO Polyclonal Antibody for WB, Indirect ELISA

Cat No. 20611-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

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Product Information

20611-1-PBS targets CENPO in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14619

  Product name: Recombinant human CENPO protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC002870

  Sequence: MEQANPLRPDGESKGGVLAHLERLETQVSRSRKQSEELQSVQAQEGALGTKIHKLRRLRDELRAVVRHRRASVKACIANVEPNQTVEINEQEALEEKLENVKAILQAYHFTGLSGKLTSRGVCVCISTAFEGNLLDSYFVDLVIQKPLRIHHHSVPVFIPLEEIAAKYLQTNIQHFLFSLCEYLNAYSGRKYQADRLQSDFAALLTGPLQRNPLCNLLSFTYKLDPGGQSFPFCARLLYKDLTATLPTDVTVTCQGVEVLSTSWEEQRASHETLFCTKPLHQVFASFTRKGEKLDMSLVS

• Full Name: centromere protein O
• Calculated Molecular Weight: 300 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC002870
• Gene Symbol: CENPO
• Gene ID (NCBI): 79172
• RRID: AB_10696179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BU64
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CENPO is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENPO may be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Besides, CENPA modulates the kinetochore-bound levels of NDC80 complex. CENPO has 2 isoforms with MW of 33 and 34 kDa, while different lysates may bind to one or two isforms in SDS-PAGE.