CENPS-CORT Polyclonal antibody

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• CENPS-CORT Polyclonal antibody (33963-1-AP)

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  WB analysis using 33963-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33963-1-AP (CENPS-CORT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CENPS-CORT Polyclonal Antibody for WB, ELISA

Cat No. 33963-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

APITD1, Apoptosis-inducing TAF9-like domain-containing protein 1, CENPS, CENP-S, CENPS-CORT Readthrough

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33963-1-AP targets CENPS-CORT in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag40952

  Product name: Recombinant human CENPS-CORT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-138 aa of

  Sequence: MEEEAETEEQQRFSYQQRLKAAVHYTVGCLCEEVALDKEMQFSKQTIAAISELTFRQCENFAKDLEMFARHAKRTTINTEDVKLLARRSNSLLKYITDKSEEIAQINLERKAQKKKKSEDGSKNSRQPAEAGVVESEN

• Full Name: CENPS-CORT readthrough
• Calculated Molecular Weight: 15kDa
• Observed Molecular Weight: 19 kDa
• Gene Symbol: CENPS-CORT
• Gene ID (NCBI): 100526739
• RRID: AB_3743126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8N2Z9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CENPS-CORT is a centromeric protein complex composed of core subunits such as CENPS and CENPT, playing a critical role in chromosome biology. This complex localizes to the centromere region and directly participates in the assembly and maintenance of the centromere-kinetochore structure, ensuring accurate chromosome segregation during both mitosis and meiosis. Its functions primarily include promoting nucleosome assembly at the centromere, maintaining the three-dimensional structural integrity of the centromere, and ensuring the fidelity of chromosome separation by regulating microtubule-kinetochore attachments. Studies have shown that dysfunction of the CENPS-CORT complex disrupts the accuracy of chromosome segregation, leading to chromosomal numerical abnormalities such as aneuploidy, which is closely associated with tumorigenesis and reproductive disorders. It is therefore an important subject in research on cell cycle regulation and genomic stability.