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CEP152 Polyclonal antibody

CEP152 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21815-1-AP

Synonyms

centrosomal protein 152kDa, Centrosomal protein of 152 kDa, CEP152, KIAA0912



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

21815-1-AP targets CEP152 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name centrosomal protein 152kDa
Calculated molecular weight 189 kDa
Observed molecular weight 110 kDa
GenBank accession numberNM_014985
Gene symbol CEP152
Gene ID (NCBI) 22995
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP152, also named as KIAA0912, is a regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. It is necessary for centrosome duplication. CEP152 functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation. CEP152, ATR and CENPJ control cellular responses to DNA damage. CEP152 has some isoforms with MW 189 kDa, 152 kDa, 110 kDa and 66 kDa.

Protocols

Product Specific Protocols
WB protocol for CEP152 antibody 21815-1-APDownload protocol
IHC protocol for CEP152 antibody 21815-1-APDownload protocol
IP protocol for CEP152 antibody 21815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Authors - I-Ling Lu
  • KD Validated