CEP250/CNAP1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 87777-1-RR (same clone as 87777-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87777-1-RR (CEP250/CNAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87777-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 87777-1-RR (same clone as 87777-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87777-1-RR (CEP250/CNAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87777-1-PBS in a different storage buffer formulation.

CEP250/CNAP1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87777-1-PBS

Clone No.253334H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Cep250, CEP250,C-NAP1, 250 kDa centrosomal protein, C NAP1, Centrosomal Nek2-associated protein 1

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Product Information

87777-1-PBS targets CEP250/CNAP1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0394

  Product name: Recombinant human CEP250,C-NAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 40-293 aa of BC001433

  Sequence: RKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLT

• Full Name: centrosomal protein 250kDa
• Calculated Molecular Weight: 281 kDa
• Observed Molecular Weight: 250-280 kDa
• GenBank Accession Number: BC001433
• Gene Symbol: CEP250/CNAP1
• Gene ID (NCBI): 11190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BV73
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.