CEP78 Polyclonal antibody

CEP78 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55341-1-AP

Synonyms

C9orf81, centrosomal protein 78kDa, Centrosomal protein of 78 kDa, CEP78, IP63

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Validation Data Gallery

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  IHC staining of human breast cancer using 55341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55341-1-AP (CEP78 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55341-1-AP (CEP78 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55341-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 55341-1-AP (CEP78 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55341-1-AP targets CEP78 in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: centrosomal protein 78kDa
• Calculated Molecular Weight: 76 kDa
• GenBank Accession Number: NM_032171
• Gene Symbol: CEP78
• Gene ID (NCBI): 84131
• RRID: AB_2881313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.