Tested Applications
| Positive WB detected in | BxPC-3 cells, human liver tissue, HuH-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15378-1-AP targets CES2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2218 Product name: Recombinant human CES2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 323-623 aa of BC032095 Sequence: LPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL Predict reactive species |
| Full Name | carboxylesterase 2 (intestine, liver) |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC032095 |
| Gene Symbol | CES2 |
| Gene ID (NCBI) | 8824 |
| RRID | AB_2077501 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00748 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CES2(Carboxylesterase 2) is also named as ICE and belongs to the type-B carboxylesterase/lipase family. Carboxylesterases (CESs) hydrolyze ester groups of drugs and toxins. CES2 specifically catalyzes the hydrolysis of the benzoyl group of cocaine and the acetyl groups of 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. CES2 differs from another liver carboxylesterase, CES1 (114835), in drug ester substrate specificity. This protein has 2 isoforms produced by alternative splicing
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CES2 antibody 15378-1-AP | Download protocol |
| WB protocol for CES2 antibody 15378-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CES2 Polyclonal antibody (Cat# 15378-1-AP) has 6 total citations across six journals: British Journal of Pharmacology, Biochemical Pharmacology, Molecular Metabolism, iScience, Drug Metabolism and Disposition, and Discover Oncology. Research fields span drug metabolism and pharmacology, cancer biology (oral squamous cell carcinoma, gastric cancer, hepatocellular carcinoma), mitochondrial dysfunction, lipid homeostasis, and protein quantification. Applications include WB, IHC, and CoIP, all in human samples.
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Icaritin enhances the antitumor efficacy of irinotecan by dual-targeting carboxylesterase 2 and p53 in vitro and in vivo. | ||
Mol Metab Carboxylesterase 2 induces mitochondrial dysfunction via disrupting lipid homeostasis in oral squamous cell carcinoma
| ||
iScience KLF13-mediated CES2 upregulation via p300-dependent acetylation sensitizes gastric cancer cells to irinotecan. | ||
Drug Metab Dispos Simultaneous absolute protein quantification of carboxylesterases 1 and 2 in human liver tissue fractions using liquid chromatography-tandem mass spectrometry. | ||
Discov Oncol Differential expression of capecitabine-related metabolic enzymes in hepatocellular carcinoma and its clinical value: a retrospective cohort study |
