CES2 Polyclonal antibody

CES2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15378-1-AP

Synonyms

Carboxylesterase 2, CE 2, CES2, CES2A1, hCE 2, iCE, PCE 2



Tested Applications

Positive WB detected inhuman stomach tissue, human liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

15378-1-AP targets CES2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CES2 fusion protein Ag2218
Full Name carboxylesterase 2 (intestine, liver)
Calculated molecular weight 62 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC032095
Gene symbol CES2
Gene ID (NCBI) 8824
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CES2(Carboxylesterase 2) is also named as ICE and belongs to the type-B carboxylesterase/lipase family. Carboxylesterases (CESs) hydrolyze ester groups of drugs and toxins. CES2 specifically catalyzes the hydrolysis of the benzoyl group of cocaine and the acetyl groups of 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. CES2 differs from another liver carboxylesterase, CES1 (114835), in drug ester substrate specificity. This protein has 2 isoforms produced by alternative splicing

Protocols

Product Specific Protocols
WB protocol for CES2 antibody 15378-1-APDownload protocol
IHC protocol for CES2 antibody 15378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Drug Metab Dispos

Simultaneous absolute protein quantification of carboxylesterases 1 and 2 in human liver tissue fractions using liquid chromatography-tandem mass spectrometry.

Authors - Sato Yuichiro Y
humanWB

Br J Pharmacol

PMID: 28128444

Authors - Wenjing Luo