CES3 Polyclonal antibody, PBS Only

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• CES3 Polyclonal antibody, PBS Only (14587-1-PBS)

• 

  WB analysis using 14587-1-AP (same clone as 14587-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14587-1-AP (CES3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14587-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 14587-1-AP (same clone as 14587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14587-1-AP (CES3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14587-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14587-1-AP (same clone as 14587-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14587-1-AP (CES3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14587-1-PBS in a different storage buffer formulation.

CES3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14587-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

UNQ869/PRO1887, ES31, EC:3.1.1.1, Carboxylesterase 3

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Product Information

14587-1-PBS targets CES3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6113

  Product name: Recombinant human CES3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 219-568 aa of BC053670

  Sequence: DLNCVTVFGGSAGGSIISGLVLSPVAAGLFHRAITQSGVITTPGIIDSHPWPLAQKIANTLACSSSSPAEMVQCLQQKEGEELVLSKKLKNTIYPLTVDGTVFPKSPKELLKEKPFHSVPFLMGVNNHEFSWLIPRGWGLLDTMEQMSREDMLAISTPVLTSLDVPPEMMPTVIDEYLGSNSDAQAKCQAFQEFMGDVFINVPTVSFSRYLRDSGSPVFFYEFQHRPSSFAKIKPAWVKADHGAEGAFVFGGPFLMDESSRLEATEEEKQLSLTMMAQWTHFARTGDPNSKALPPWPQFNQAEQYLEINPVPRAGQKFREAWMQFWSETLPSKIQQWHQKQKNRKAQEDL

• Full Name: carboxylesterase 3
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 60-62 kDa
• GenBank Accession Number: BC053670
• Gene Symbol: CES3
• Gene ID (NCBI): 23491
• RRID: AB_2275992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UWW8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It is expressed in liver, colon and small intestine. This antibody recognizes both isoforms with MW 60-62 kDa and 24 kDa.