Cingulin Polyclonal antibody

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Cingulin Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21369-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CGN, KIAA1319

• 

  WB analysis using 21369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 21369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 21369-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse small intestine using 21369-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 21369-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse kidney using 21369-1-AP

  IP result of anti-Cingulin (IP:21369-1-AP, 4ug; Detection:21369-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 21369-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21369-1-AP (Cingulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293T cells, A431 cells, MCF-7 cells, mouse kidney tissue, mouse small intestine tissue, Jurkat cells, mouse colon tissue, HeLa cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21369-1-AP targets Cingulin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15843

  Product name: Recombinant human CGN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC146657

  Sequence: MAEPRGPVDHGVQIRFITEPVSGAEMGTLRRGGRRPAKDARASTYGVAVRVQGIAGQPFVVLNSGEKGGDSFGVQIKGANDQGASGALSSDLELPENPYSQVKGFPAPSQSSTSDEEPGAYWNGKLLRSHSQASLAGPGPVDPSNRSNSMLELAPKVASPGSTIDTAPLSSVDSLINKFDSQLGGQARGRTGRRTRMLPPEQRKRSKSLDSRLPRDTFEERERQSTNHWTSSTKYDNHVGTSKQPAQSQNLSPLSGFSRSRQTQDWVLQSFEEPRRSAQDPTMLQFKSTPDLLRDQQEAAPPGSVDHMKATIYGILREGSSESETSVRRKVSLVLEKMQPLVMVSSGSTK

• Full Name: cingulin
• Calculated Molecular Weight: 1197 aa, 136 kDa
• Observed Molecular Weight: 136-150 kDa
• GenBank Accession Number: BC146657
• Gene Symbol: Cingulin
• Gene ID (NCBI): 57530
• RRID: AB_10733647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2M7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier.

Publications

What published studies show

The anti-Cingulin antibody (Cat# 21369-1-AP) has 13 citations across journals including Nature Communications, EMBO Molecular Medicine, Cell Molecular Gastroenterology and Hepatology, Journal of Genetics and Genomics, Genomics Proteomics & Bioinformatics, Mucosal Immunology, Scientific Reports, Cancers, Cell Signaling, J Cell Mol Med, Cardiovascular Toxicology, and Biology of Reproduction. Research fields span epithelial polarization, circadian rhythms, intestinal barrier integrity, hearing, colorectal cancer, hypertension, myocardial infarction, and testis biology.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
canine	WB,IF	Nat Commun; Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.; Authors - Anthony J Mangan ; KO Validated
human	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
		Cell Mol Gastroenterol Hepatol; Single Immunoglobulin Interleukin1-Related Receptor-TWIST1 Axis Regulates Barrier Function in Neonatal Intestine.; Authors - Aparna Venkatraman
human,canine	WB,IF	J Genet Genomics; A human-specific cytotoxic neopeptide generated by the deafness gene Cingulin; Authors - Yuhang Huang
mouse,human,canine	WB,IF	EMBO Mol Med; Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse; Authors - Guang-Jie Zhu
human	WB	Genomics Proteomics Bioinformatics; Integrative Omics Uncovers Low Tumorous Magnesium Content as A Driver Factor of Colorectal Cancer; Authors - Rou Zhang