Cingulin Polyclonal antibody

• Featured Product
• KD/KO Validated

Cingulin Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 21369-1-AP

Synonyms

KIAA1319, CGN

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Validation Data Gallery

• 

  WB analysis using 21369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21369-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21369-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21369-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 21369-1-AP

  IP result of anti-Cingulin (IP:21369-1-AP, 4ug; Detection:21369-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

• 

  IHC staining of human ovary cancer using 21369-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21369-1-AP (Cingulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse kidney tissue, mouse small intestine tissue, MCF-7 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

21369-1-AP targets Cingulin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cingulin fusion protein Ag15843
• Full Name: cingulin
• Calculated Molecular Weight: 1197 aa, 136 kDa
• Observed Molecular Weight: 136-150 kDa
• GenBank Accession Number: BC146657
• Gene Symbol: Cingulin
• Gene ID (NCBI): 57530
• RRID: AB_10733647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier.

Publications

Species	Application	Title
human	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
canine	WB,IF	Nat Commun; Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.; Authors - Anthony J Mangan ; KO Validated
mouse	WB,IF	Mucosal Immunol; Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.; Authors - Jiayao Ou
human	WB,IHC	Cancers (Basel); miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN.; Authors - Xiaohui Zhang
rat	WB	Sci Rep; Treatment with Ligilactobacillus murinus lowers blood pressure and intestinal permeability in spontaneously hypertensive rats; Authors - Masashi Mukohda
mouse	WB, IP	Cell Signal; Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate.; Authors - Serge Ducommun

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