CHAC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

30

Cat no : 15207-1-AP

Synonyms

Glutathione-specific gamma-glutamylcyclotransferase 1, Gamma-GCG 1, EC:4.3.2.7, Cation transport regulator-like protein 1, Botch

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Validation Data Gallery

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  WB analysis using 15207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15207-1-AP

  WB result of CHAC1 antibody (15207-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHAC1 transfected HeLa cells.

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  IHC staining of human stomach using 15207-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15207-1-AP (CHAC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 15207-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15207-1-AP (CHAC1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, CHO cells, U-251 cells, C6 cells, RAW 264.7 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Product Information

15207-1-AP targets CHAC1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

• Tested Reactivity: human, mouse, rat, hamster
• Cited Reactivity: human, mouse, rat, chicken, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHAC1 fusion protein Ag7360
• Full Name: ChaC, cation transport regulator homolog 1 (E. coli)
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC001847
• Gene Symbol: CHAC1
• Gene ID (NCBI): 79094
• RRID: AB_2878118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHAC1(Cation transport regulator-like protein 1) was identified in a co-regulated group of genes enriched for components of the ATF4 (activating transcription factor 4) arm of the unfolded protein response pathway. CHAC1 is a proapoptotic ER stress protein downstream of the pancreatic EIF2α kinase-ATF4 pathway that appears to be important for human physiology and disease(PMID: 19109178). In addition, the study found that high CHAC1 expression is associated with a bad prognosis hinting that CHAC1 may have a possible prognostic significance in breast cancer(PMID: 35930144). The predicted molecular weight of CHAC1 is 24 kDa, and the molecular weight detected by Proteintech is 38 kDa.

Publications

Species	Application	Title
	WB	Proc Natl Acad Sci U S A; Consequences of poly(ethylene oxide) and poloxamer P188 on transcription in healthy and stressed myoblasts; Authors - Adelyn A Crabtree
mouse	WB	Cell Mol Gastroenterol Hepatol; Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis.; Authors - Sha Huang
human	WB	Free Radic Biol Med; High-fat diet aggravates colitis-associated carcinogenesis by evading ferroptosis in the ER stress-mediated pathway.; Authors - Xiaoli Zhang
human	WB	Lung; ETS1 Ameliorates Hyperoxia-Induced Bronchopulmonary Dysplasia in Mice by Activating Nrf2/HO-1 Mediated Ferroptosis; Authors - Min Yang
human	WB	Front Oncol; Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer; Authors - Pengyu Fan
human	IHC	J Cell Mol Med; Ferroptosis-related gene NOX4, CHAC1 and HIF1A are valid biomarkers for stomach adenocarcinoma.; Authors - Ruoxi Xiao