Tested Applications
| Positive WB detected in | Jurkat cells, Raji cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16304-1-AP targets CHAC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9281 Product name: Recombinant human CHAC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC019239 Sequence: MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPAGCVWGVAYRLPVGKEEEVKAYLDFREKGGYRTTTVIFYPKDPTTKPFSVLLYIGTCDNPDYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELANSIRNLVPEEADEHLFALEKLVKERLEGKQNLNCI Predict reactive species |
| Full Name | ChaC, cation transport regulator homolog 2 (E. coli) |
| Calculated Molecular Weight | 184 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC019239 |
| Gene Symbol | CHAC2 |
| Gene ID (NCBI) | 494143 |
| RRID | AB_2229405 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WUX2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHAC2 is a gamma-glutamyl cyclotransferase that catalyzes the conversion of glutathione to 5-oxoproline and cysteinylglycine. CHAC2 is involved in continuous, basal turnover of cytosolic glutathione(PMID: 27913623).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHAC2 antibody 16304-1-AP | Download protocol |
| WB protocol for CHAC2 antibody 16304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CHAC2 antibody (Cat# 16304-1-AP) has 8 total citations. Citations appear in journals including International Journal of Oral Science, Cancer Letters, International Journal of Biological Sciences, Cells, Molecular Neurobiology, Journal of Cancer, Environmental Toxicology, and Journal of Molecular Histology. Research fields span inflammatory and immune regulation, gastric and lung cancer, cardiorenal syndrome, infectious disease, neurology (Lennox-Gastaut syndrome), and acute liver injury.
| Species | Application | Title |
|---|---|---|
Int J Oral Sci CHAC2-mediated glutathione metabolic reprogramming drives N1 polarization of bone marrow neutrophils and exacerbates inflammatory comorbidities. | ||
Cancer Lett Hypoxia-driven phase separation of the PABP1/eIF4B complex forms stress granules and activates ChaC2 translation to promote polyunsaturated lipids-supported peritoneal metastasis in gastric cancer. | ||
Int J Biol Sci Febuxostat therapy improved the outcomes of cardiorenal syndrome rodent through alleviating xanthine oxidase-induced oxidative stress and mitochondrial dysfunction | ||
Cells Adh Promotes Actinobacillus pleuropneumoniae Survival in Porcine Alveolar Macrophages by Inhibiting CHAC2-Mediated Respiratory Burst and Inflammatory Cytokine Expression | ||
Mol Neurobiol NUS1 Variants Cause Lennox-Gastaut Syndrome Related to Unfolded Protein Reaction Activation | ||
J Cancer CHAC2 promotes lung adenocarcinoma by regulating ROS-mediated MAPK pathway activation
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