CHCHD6 Polyclonal antibody

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• CHCHD6 Polyclonal antibody (20639-1-AP)

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• KD/KO Validated

CHCHD6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20639-1-AP Copied!

Publications (20)

Reviews (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CHCM1, Coiled-coil-helix cristae morphology protein 1, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, MIC25, MICOS complex subunit MIC25

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  WB analysis using 20639-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20639-1-AP (CHCHD6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Jurkat using 20639-1-AP

  IP result of anti-CHCHD6 (IP:20639-1-AP, 4ug; Detection:20639-1-AP 1:2000) with Jurkat cells lysate 1560 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 20639-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20639-1-AP (CHCHD6 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 20639-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20639-1-AP (CHCHD6 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 20639-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CHCHD6 antibody (20639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20639-1-AP targets CHCHD6 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14700

  Product name: Recombinant human CHCHD6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-235 aa of BC006123

  Sequence: MGSTESSEGRRVSFGVDEEERVRVLQGVRLSENVVNRMKEPSSPPPAPTSSTFGLQDGNLRAPHKESTLPRSGSSGGQQPSGMKEGVKRYEQEHAAIQDKLFQVAKREREAATKHSKASLPTGEGSISHEEQKSVRLARELESREAELRRRDTFYKEQLERIERKNAEMYKLSSEQFHEAASKMESTIKPRRVEPVCSGLQAQILHCYRDRPHEVLLCSDLVKAYQRCVSAAHKG

• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 6
• Calculated Molecular Weight: 235 aa, 26 kDa
• Observed Molecular Weight: 26-29 kDa
• GenBank Accession Number: BC006123
• Gene Symbol: CHCHD6
• Gene ID (NCBI): 84303
• RRID: AB_10697667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BRQ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

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Automatically generated from the 20 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	CoIP	Sci Bull (Beijing); Mitochondria serve as a source of mineral precursors initiating early cartilage calcification in osteoarthritis.; Authors - Qianqian Wan
human	WB	Mol Cell; BRD4-mediated ER membrane contact creates functionally distinct mitochondrial subtypes.; Authors - Brandon Chen
human	WB	Adv Sci (Weinh); LRRC4 Orchestrates AP2A1-Containing Clathrin-Coated Vesicles to Disrupt Mitochondrial Cristae and Restrict Glioblastoma Progression; Authors - Yang Li
mouse	WB	Cell Death Differ; Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.; Authors - H Li ; KD Validated
mouse	WB,IF	Acta Neuropathol; A CHCHD6-APP axis connects amyloid and mitochondrial pathology in Alzheimer's disease; Authors - Yutong Shang ; KO Validated
human	WB	J Cell Biol; The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy; Authors - Mehmet Oguz Gok

Reviews

4.7 out of 5

4 verified customer reviews

What customers say

All four reviews are positive, with an average rating of 4.75 out of 5. Users consistently report clear, specific bands in Western Blot applications at a 1:1000 dilution. Tested tissues include mouse heart, human heart, and cancer tissue. Customers describe the antibody as "very good" and note it "worked very well." No negative feedback or issues were reported across any of the reviews.

Automatically generated from the 4 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Angela (Verified Customer) (12-27-2024)

good

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart tissue

CHCHD6 Antibody Western Blot validation (1:1000 dilution) in heart tissue (Cat no:20639-1-AP)

angela (Verified Customer) (12-27-2024)

very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: heart tissue

CHCHD6 Antibody Western Blot validation (1;1000 dilution) in heart tissue (Cat no:20639-1-AP)

SITING (Verified Customer) (07-13-2020)

very clear band in our wester blot expose result

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MOUSE HEART

Jingwen (Verified Customer) (01-28-2020)

This antibody worked very well when I performed western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer tissue