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CHCHD6 Polyclonal antibody
CHCHD6 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 20639-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
Product Information
The immunogen of 20639-1-AP is CHCHD6 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CHCHD6 fusion protein Ag14700 |
Full Name | coiled-coil-helix-coiled-coil-helix domain containing 6 |
Calculated molecular weight | 235 aa, 26 kDa |
Observed molecular weight | 26-29 kDa |
GenBank accession number | BC006123 |
Gene symbol | CHCHD6 |
Gene ID (NCBI) | 84303 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
IHC protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
IP protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Life Sci Alliance MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes. | ||
Sci Rep Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.
| ||
Cell Death Differ Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.
| ||
Elife QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease. | ||
EMBO Rep Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner. | ||
Elife QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH SITING (Verified Customer) (07-13-2020) | very clear band in our wester blot expose result
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FH Jingwen (Verified Customer) (01-28-2020) | This antibody worked very well when I performed western blot.
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