CHCHD6 Polyclonal antibody

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  WB analysis using 20639-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20639-1-AP (CHCHD6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 20639-1-AP

  IP result of anti-CHCHD6 (IP:20639-1-AP, 4ug; Detection:20639-1-AP 1:2000) with Jurkat cells lysate 1560 ug.

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  IHC staining of human stomach using 20639-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20639-1-AP (CHCHD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach using 20639-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20639-1-AP (CHCHD6 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of MCF-7 using 20639-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CHCHD6 antibody (20639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CHCHD6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20639-1-AP

Publications(15)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MICOS complex subunit MIC25, MIC25, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, Coiled-coil-helix cristae morphology protein 1, CHCM1

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IF: See 1 publications below

Product Information

20639-1-AP targets CHCHD6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHCHD6 fusion protein Ag14700
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 6
• Calculated Molecular Weight: 235 aa, 26 kDa
• Observed Molecular Weight: 26-29 kDa
• GenBank Accession Number: BC006123
• Gene Symbol: CHCHD6
• Gene ID (NCBI): 84303
• RRID: AB_10697667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BRQ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Elife; QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.; Authors - Virginia Guarani
human	WB	EMBO Rep; Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.; Authors - Arun Kumar Kondadi
human	WB	Elife; QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease.; Authors - Virginia Guarani
mouse	WB	Cell Death Differ; Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.; Authors - H Li ; KD Validated
human	WB	Sci Rep; Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.; Authors - Chengli Ding ; KO Validated
human	WB	Biochim Biophys Acta Biomembr; Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes.; Authors - Jennifer Urbach

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angela (Verified Customer) (12-27-2024)

good

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart tissue

CHCHD6 Antibody Western Blot validation (1:1000 dilution) in heart tissue (Cat no:20639-1-AP)

FH

angela (Verified Customer) (12-27-2024)

very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: heart tissue

CHCHD6 Antibody Western Blot validation (1;1000 dilution) in heart tissue (Cat no:20639-1-AP)

FH

SITING (Verified Customer) (07-13-2020)

very clear band in our wester blot expose result

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MOUSE HEART

FH

Jingwen (Verified Customer) (01-28-2020)

This antibody worked very well when I performed western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer tissue