CHCHD7 Polyclonal antibody

CHCHD7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19911-1-AP

Synonyms

CHCHD7

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Validation Data Gallery

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  WB analysis of mouse pancreas using 19911-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19911-1-AP (CHCHD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 19911-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19911-1-AP (CHCHD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 19911-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19911-1-AP (CHCHD7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 19911-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19911-1-AP (CHCHD7 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, human placenta tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19911-1-AP is CHCHD7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHCHD7 fusion protein Ag13735
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 7
• Calculated Molecular Weight: 97 aa, 12 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC002546
• Gene Symbol: CHCHD7
• Gene ID (NCBI): 79145
• RRID: AB_10666734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.