CHD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHD1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20576-1-AP

Synonyms

ATP dependent helicase CHD1, CHD 1, CHD1

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Validation Data Gallery

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  WB analysis of HeLa using 20576-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20576-1-AP (CHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20576-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20576-1-AP (CHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20576-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20576-1-AP (CHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20576-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20576-1-AP (CHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20576-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20576-1-AP (CHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20576-1-AP

  IP result of anti-CHD1 (IP:20576-1-AP, 4ug; Detection:20576-1-AP 1:300) with HeLa cells lysate 1600ug.

• 

  IP experiment of HeLa using 20576-1-AP

  IP result of anti-CHD1 (IP:20576-1-AP, 4ug; Detection:20576-1-AP 1:300) with HeLa cells lysate 1600ug.

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, human brain tissue, Jurkat cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

20576-1-AP targets CHD1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chromodomain helicase DNA binding protein 1
• Calculated Molecular Weight: 197 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_001270
• Gene Symbol: CHD1
• Gene ID (NCBI): 1105
• RRID: AB_10694288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHD1 belongs to the SNF2/RAD54 helicase family. CHD1 is an aTP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. CHD1 regulates polymerase II transcription. It is required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. CHD1 regulates negatively DNA replication. It is required to maintain a specific chromatin configuration across the genome. It is associated with histone deacetylase (HDAC) activity. CHD1 is required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. It functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. It is required for maintaining open chromatin and pluripotency in embryonic stem cells. In PTEN-deficient prostate and breast cancers, CHD1 depletion profoundly and specifically suppressed cell proliferation, cell survival and tumorigenic potential. The antibody is specific to CHD1.

Publications

Species	Application	Title
human	WB	EMBO J; Chromatin remodeler CHD1 promotes XPC-to-TFIIH handover of nucleosomal UV lesions in nucleotide excision repair.; Authors - Peter Rüthemann ; KD Validated
human	WB	Cell Prolif; MATN1-AS1 promotes glioma progression by functioning as ceRNA of miR-200b/c/429 to regulate CHD1 expression.; Authors - Jun Zhu
mouse	WB	Front Behav Neurosci; Impaired Contextual Fear Extinction Learning is Associated with Aberrant Regulation of CHD-Type Chromatin Remodeling Factors.; Authors - Alexandra Wille
		Behav Brain Res; Dysregulation of select ATP-dependent chromatin remodeling factors in high trait anxiety.; Authors - Alexandra Wille
mouse	WB, IF	Sci Rep; Embryonic stem cell differentiation requires full length Chd1.; Authors - Paolo Piatti
mouse	WB	Front Mol Neurosci; Role for Chromatin Remodeling Factor Chd1 in Learning and Memory.; Authors - Ines Schoberleitner