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  • KD/KO Validated

CHD1 Polyclonal antibody

CHD1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 20576-1-AP

Synonyms

ATP dependent helicase CHD1, CHD 1, CHD1



Tested Applications

Positive WB detected inHeLa cells, A431 cells, human brain tissue, Jurkat cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20576-1-AP targets CHD1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name chromodomain helicase DNA binding protein 1
Calculated Molecular Weight 197 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberNM_001270
Gene Symbol CHD1
Gene ID (NCBI) 1105
RRIDAB_10694288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHD1 belongs to the SNF2/RAD54 helicase family. CHD1 is an aTP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. CHD1 regulates polymerase II transcription. It is required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. CHD1 regulates negatively DNA replication. It is required to maintain a specific chromatin configuration across the genome. It is associated with histone deacetylase (HDAC) activity. CHD1 is required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. It functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. It is required for maintaining open chromatin and pluripotency in embryonic stem cells. In PTEN-deficient prostate and breast cancers, CHD1 depletion profoundly and specifically suppressed cell proliferation, cell survival and tumorigenic potential. The antibody is specific to CHD1.

Protocols

Product Specific Protocols
WB protocol for CHD1 antibody 20576-1-APDownload protocol
IP protocol for CHD1 antibody 20576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

Chromatin remodeler CHD1 promotes XPC-to-TFIIH handover of nucleosomal UV lesions in nucleotide excision repair.

Authors - Peter Rüthemann
  • KD Validated
humanWB

Cell Prolif

MATN1-AS1 promotes glioma progression by functioning as ceRNA of miR-200b/c/429 to regulate CHD1 expression.

Authors - Jun Zhu
mouseWB

Front Behav Neurosci

Impaired Contextual Fear Extinction Learning is Associated with Aberrant Regulation of CHD-Type Chromatin Remodeling Factors.

Authors - Alexandra Wille

Behav Brain Res

Dysregulation of select ATP-dependent chromatin remodeling factors in high trait anxiety.

Authors - Alexandra Wille
mouseWB, IF

Sci Rep

Embryonic stem cell differentiation requires full length Chd1.

Authors - Paolo Piatti
mouseWB

Front Mol Neurosci

Role for Chromatin Remodeling Factor Chd1 in Learning and Memory.

Authors - Ines Schoberleitner