Tested Applications
| Positive WB detected in | HepG2 cells, multi-cells/tissue |
| Positive IHC detected in | human endometrial cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12169-1-AP targets CHFR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2811 Product name: Recombinant human CHFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 344-652 aa of BC012072 Sequence: NNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN Predict reactive species |
| Full Name | checkpoint with forkhead and ring finger domains |
| Calculated Molecular Weight | 664 aa, 73 kDa |
| Observed Molecular Weight | 70-85 kDa |
| GenBank Accession Number | BC012072 |
| Gene Symbol | CHFR |
| Gene ID (NCBI) | 55743 |
| RRID | AB_2080821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96EP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Checkpoint with forkhead and RING finger domains protein(CHFR) is a checkpoint protein that reportedly initiates a cell cycle delay in response to microtubule stress during prophase in mitosis and it has been hypothesized to be a tumor suppressor with a potential role as a biomarker for chemotherapeutic response to Taxol(PMID:17596595). It belongs to the CHFR family. Endogenous Chfr is ubiquitinated and phosphorylated in an MPF-dependent manner in mitotic Xenopus egg extracts(PMID:18504434). It has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CHFR antibody 12169-1-AP | Download protocol |
| WB protocol for CHFR antibody 12169-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHFR antibody (catalog no. 12169-1-AP) has 9 total citations. These appear in journals including Nature Communications, Clinical Cancer Research, Molecular Medicine Reports, Translational Oncology, Journal of Biological Chemistry, Frontiers in Genetics, bioRxiv, and Biology Direct. The associated research fields span cancer biology (colon, breast, gastric, renal cell carcinoma, and leukemia), cell signaling, DNA repair, and endothelial biology, with applications mainly in Western blot and IHC.
| Species | Application | Title |
|---|---|---|
Nat Commun Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions
| ||
Clin Cancer Res Association of CHFR promoter methylation with disease recurrence in locally advanced colon cancer. | ||
Mol Med Rep CHFR‑mediated epithelial‑to‑mesenchymal transition promotes metastasis in human breast cancer cells.
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J Biol Chem CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation. | ||
Front Genet Identification of a Novel Epigenetic Signature CHFR as a Potential Prognostic Gene Involved in Metastatic Clear Cell Renal Cell Carcinoma.
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Reviews
What customers say
Both reviewers rated this antibody 5 stars. Users report excellent performance across Western Blot, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation. Recommended dilutions were 1:500–1:1000 depending on the application. One user highlighted sharp, bright, and accurate bands, while another confirmed reliable results in colon cancer cells. Overall, the antibody is well-regarded for its specificity and consistent signal quality across multiple techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (01-08-2026) | these antibodies are excellent, bands and its brightness are accurate!
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Iram (Verified Customer) (02-11-2022) | This antibody works for me great
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