CHFR Polyclonal antibody

• Featured Product
• KD/KO Validated

CHFR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 12169-1-AP

Synonyms

CHFR, RING finger protein 196, RNF116, RNF196

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Validation Data Gallery

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  WB analysis of HepG2 using 12169-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells/tissue using 12169-1-AP

  WB result of 12169-1-AP (CHFR antibody).

• 

  WB analysis of SMMC-7721 using 12169-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12169-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12169-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12169-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 12169-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12169-1-AP (CHFR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 12169-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12169-1-AP (CHFR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 12169-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12169-1-AP (CHFR antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, multi-cells/tissue
• Positive IHC detected in: human endometrial cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

12169-1-AP targets CHFR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHFR fusion protein Ag2811
• Full Name: checkpoint with forkhead and ring finger domains
• Calculated Molecular Weight: 664 aa, 73 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC012072
• Gene Symbol: CHFR
• Gene ID (NCBI): 55743
• RRID: AB_2080821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Checkpoint with forkhead and RING finger domains protein(CHFR) is a checkpoint protein that reportedly initiates a cell cycle delay in response to microtubule stress during prophase in mitosis and it has been hypothesized to be a tumor suppressor with a potential role as a biomarker for chemotherapeutic response to Taxol(PMID:17596595). It belongs to the CHFR family. Endogenous Chfr is ubiquitinated and phosphorylated in an MPF-dependent manner in mitotic Xenopus egg extracts(PMID:18504434). It has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IHC	Clin Cancer Res; Association of CHFR promoter methylation with disease recurrence in locally advanced colon cancer.; Authors - Tanaka Motofumi M
human	WB	Transl Oncol; Silencing of CHFR Sensitizes Gastric Carcinoma to PARP Inhibitor Treatment.; Authors - Yuan Li
human	IHC	J Biol Chem; CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.; Authors - Kashima Lisa L
human	WB	Front Genet; Identification of a Novel Epigenetic Signature CHFR as a Potential Prognostic Gene Involved in Metastatic Clear Cell Renal Cell Carcinoma.; Authors - Xiangling Chen ; KD Validated
human	WB	Mol Med Rep; CHFR‑mediated epithelial‑to‑mesenchymal transition promotes metastasis in human breast cancer cells.; Authors - Guobin Jiang ; KD Validated
human,mouse	WB	Nat Commun; Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions; Authors - Chinnaswamy Tiruppathi ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (02-11-2022)

This antibody works for me great

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:500 for IF
• Cell Tissue Type: Colon cancer cells