Tested Applications
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human stomach tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21484-1-AP targets CHIA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15767 Product name: Recombinant human CHIA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-368 aa of BC036339 Sequence: MVSTPENRQTFITSVIKFLRQYEFDGLDFDWEYPGSRGSPPQDKHLFTVLVQEMREAFEQEAKQINKPRLMVTAAVAAGISNIQSGYEIPQLSQYLDYIHVMTYDLHGSWEGYTGENSPLYKYPTDTGSNAYLNVDYVMNYWKDNGAPAEKLIVGFPTYGHNFILSNPSNTGIGAPTSGAGPAGPYAKESGIWAYYEICTFLKNGATQGWDAPQEVPYAYQGNVWVGYDNIKSFDIKAQWLKHNKFGGAMVWAIDLDDFTGTFCNQGKFPLISTLKKALGLQSASCTAPAQPIEPITAAPSGSGNGSGSSSSGGSSGGSGFCAVRANGLYPVANNRNAFWHCVNGVTYQQNCQAGLVFDTSCDCCNWA Predict reactive species |
| Full Name | chitinase, acidic |
| Calculated Molecular Weight | 476 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC036339 |
| Gene Symbol | CHIA |
| Gene ID (NCBI) | 27159 |
| RRID | AB_10755301 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZP6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHIA (Chitinase Acidic), also known as AMCase or CHIT2, is a member of the 18-glycosidase family that functions optimally under acidic conditions. CHIA is a bifunctional enzyme with both immunological and digestive roles. Its N-terminal catalytic domain hydrolyzes chitin, a polymer of N-acetylglucosamine found in fungal cell walls, arthropod exoskeletons, and nematodes. CHIA expression is markedly altered in rhinitis, conjunctivitis, chronic atrophic gastritis, and type 2 diabetes, and it serves as a biomolecular marker for chronic atrophic gastritis and sepsis-associated acute kidney injury.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHIA antibody 21484-1-AP | Download protocol |
| IHC protocol for CHIA antibody 21484-1-AP | Download protocol |
| WB protocol for CHIA antibody 21484-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHIA Polyclonal antibody (Cat# 21484-1-AP) has 4 total citations. These appear in Communications Biology, International Immunopharmacology, Frontiers in Molecular Biosciences, and European Journal of Immunology. The associated research fields span gastric cell stress responses, fracture-induced lung inflammation, atherosclerosis aging signatures, and fungal corneal infection immunology. Applications cited include immunofluorescence and Western blot in mouse and human samples.
| Species | Application | Title |
|---|---|---|
Commun Biol Elevated stress response marks deeply quiescent reserve cells of gastric chief cells | ||
Int Immunopharmacol miR146a/circKMT2c regulates inflammation in fracture-induced acute lung injury via p65 nuclear translocation | ||
Front Mol Biosci Characterization of an Aging-Based Diagnostic Gene Signature and Molecular Subtypes With Diverse Immune Infiltrations in Atherosclerosis. | ||
Eur J Immunol Aspergillus fumigatus corneal infection is regulated by chitin synthases and by neutrophil-derived acidic mammalian chitinase. |
