Chk1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 80056-2-RR (same clone as 80056-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80056-2-RR (Chk1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80056-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293T using 80056-2-RR (same clone as 80056-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using Chk1 antibody (80056-2-RR, Clone: 2G7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80056-2-PBS in a different storage buffer formulation.

Chk1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 80056-2-PBS

Clone No.2G7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CHEK1, 2G7, Checkpoint kinase-1, CHEK 1, Chk 1

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Product Information

80056-2-PBS targets Chk1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: CHK1 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC004202
• Gene Symbol: Chk1
• Gene ID (NCBI): 1111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O14757
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.