Tested Applications
| Positive WB detected in | COLO 320 cells, mouse colon tissue, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13520-1-AP targets Choline Kinase Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4761 Product name: Recombinant human CHKA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 139-439 aa of BC036471 Sequence: EPRKVLLRLYGAILQVGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELGLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGQWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV Predict reactive species |
| Full Name | choline kinase alpha |
| Calculated Molecular Weight | 439 aa, 52 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC036471 |
| Gene Symbol | CHKA |
| Gene ID (NCBI) | 1119 |
| RRID | AB_2079160 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35790 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline phosphotransferase) is a cytosolic enzyme that catalyzes the committed step in the synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the phosphorylation of choline with ATP to form phosphocholine and ADP(PMID:9506987). It has 2 isoforms produced by alternative splicing with the molecular weight of 52 kDa and 50 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Choline Kinase Alpha antibody 13520-1-AP | Download protocol |
| IHC protocol for Choline Kinase Alpha antibody 13520-1-AP | Download protocol |
| IP protocol for Choline Kinase Alpha antibody 13520-1-AP | Download protocol |
| WB protocol for Choline Kinase Alpha antibody 13520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Choline Kinase Alpha Polyclonal antibody (Cat# 13520-1-AP) has 27 citations in journals including Cell Metabolism, Nature Communications, PNAS, Annals of the Rheumatic Diseases, Clinical Cancer Research, Journal of Biological Chemistry, Cell Death & Disease, Cancer Letters, and PLoS ONE. Research fields include choline and lipid metabolism, various cancers (breast, ovarian, prostate, gastric, cervical, endometrial, hepatocellular), rheumatoid arthritis, inflammatory bowel disease, liver injury, atherosclerosis, preeclampsia, and sepsis.
| Species | Application | Title |
|---|---|---|
Nat Commun Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis. | ||
Cell Death Dis DHRS2 inhibits cell growth and metastasis in ovarian cancer by downregulation of CHKα to disrupt choline metabolism | ||
Phytomedicine Integrated metabolomics and lipidomics investigation of the mechanism of Danggui Sini Decoction on improving lipid homeostasis in primary dysmenorrhea |
Reviews
What customers say
The single review for this product is negative (1/5). The user performed a Western Blot on mouse liver and spleen tissue at a 1:500 dilution and reported that the antibody did not perform as expected based on the datasheet. They expressed surprise at the result and questioned whether the issue was specific to that particular lot. No further details or troubleshooting steps were provided in the review.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (02-02-2021) | I was surprised it did not work as shown in the datasheet. It is possible jus this batch?
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