Choline Kinase Alpha Polyclonal antibody

• Featured Product
• KD/KO Validated

Choline Kinase Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (1)

Cat no : 13520-1-AP

Synonyms

CHKA, EC:2.7.1.82, EC:2.7.1.32, CKI, CHK

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Validation Data Gallery

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  WB analysis using 13520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13520-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 13520-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 13520-1-AP

  IP result of anti-Choline Kinase Alpha (IP:13520-1-AP, 4ug; Detection:13520-1-AP 1:500) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human liver cancer using 13520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 13520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13520-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Choline Kinase Alpha antibody (13520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13520-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13520-1-AP (Choline kinase alpha antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, COLO 320 cells, mouse colon tissue, rat spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• ELISA: See 1 publications below

Product Information

13520-1-AP targets Choline Kinase Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Choline Kinase Alpha fusion protein Ag4761
• Full Name: choline kinase alpha
• Calculated Molecular Weight: 439 aa, 52 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC036471
• Gene Symbol: CHKA
• Gene ID (NCBI): 1119
• RRID: AB_2079160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline phosphotransferase) is a cytosolic enzyme that catalyzes the committed step in the synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the phosphorylation of choline with ATP to form phosphocholine and ADP(PMID:9506987). It has 2 isoforms produced by alternative splicing with the molecular weight of 52 kDa and 50 kDa.

Publications

Species	Application	Title
human	WB,IHC	Proc Natl Acad Sci U S A; The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation; Authors - Qingxia Ma
mouse	WB, IHC	Ann Rheum Dis; Choline kinase inhibition in rheumatoid arthritis.; Authors - M Guma
human	WB	Clin Cancer Res; Preclinical development of a non-toxic oral formulation of monoethanolamine, a lipid precursor, for prostate cancer treatment.; Authors - Roopali Saxena
mouse	WB	Front Cell Dev Biol; Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration.; Authors - Ming Kong
rat	WB	J Biol Chem; ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.; Authors - Arsenault Daniel J DJ
	WB	Mol Biol Cell; Nuclear-localized CTP:phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis.; Authors - Adam J Aitchison

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (02-02-2021)

I was surprised it did not work as shown in the datasheet. It is possible jus this batch?

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse liver and mouse spleen

Choline Kinase Alpha Antibody Western Blot validation (1:500 dilution) in mouse liver and mouse spleen (Cat no:13520-1-AP)