Choline Kinase Alpha Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 84881-2-RR (same clone as 84881-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84881-2-RR (CHKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84881-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01644-2

  Cytometric bead array standard curve of MP01644-2, CHKA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84881-1-PBS. Detection antibody: 84881-2-PBS. Standard: Ag4761. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 84881-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84881-2-RR against Human CHKA were performed. The affinity constant is below 1 pM.

Choline Kinase Alpha Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 84881-2-PBS

Clone No.242095B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

CHKA, 242095B4, CHETK-alpha, CHK, CKI

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Product Information

84881-2-PBS targets Choline Kinase Alpha as part of a matched antibody pair:

MP01644-2: 84881-1-PBS capture and 84881-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4761

  Product name: Recombinant human CHKA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 139-439 aa of BC036471

  Sequence: EPRKVLLRLYGAILQVGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELGLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGQWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV

• Full Name: choline kinase alpha
• Calculated Molecular Weight: 439 aa, 52 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC036471
• Gene Symbol: CHKA
• Gene ID (NCBI): 1119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35790
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.