CHMP1B Polyclonal antibody

CHMP1B Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14639-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Chromatin-modifying protein 1b, chromatin modifying protein 1B, CHMP1.5, C18orf2, C18 ORF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA431 cells, human heart tissue, mouse heart tissue, rat heart tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14639-1-AP targets CHMP1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6222

Product name:

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: of

Sequence:

Predict reactive species
Full Name chromatin modifying protein 1B
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC065933
Gene Symbol CHMP1B
Gene ID (NCBI) 57132
RRIDAB_2079353
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7LBR1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHMP1B antibody 14639-1-APDownload protocol
IHC protocol for CHMP1B antibody 14639-1-APDownload protocol
IP protocol for CHMP1B antibody 14639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Science

Triggered recruitment of ESCRT machinery promotes endolysosomal repair.

Authors - Michael L Skowyra
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
humanWB

J Extracell Vesicles

Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation

Authors - Pauline P Marie
humanIF, WB

Dev Cell

NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.

Authors - Oliver B Davis
humanIF

Elife (Cambridge)

Structure of cellular ESCRT-III spirals and their relationship to HIV budding.

Authors - Anil G Cashikar
human,mouseIF

MBio

Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.

Authors - Ekansh Mittal
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