CHMP1B Polyclonal antibody

CHMP1B Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14639-1-AP

Synonyms

C10orf2, C18 ORF2, C18orf2, CHMP1.5, CHMP1B, chromatin modifying protein 1B, hVps46 2, Vps46 2



Tested Applications

Positive WB detected inhuman heart tissue, A431 cells, HeLa cells, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14639-1-AP targets CHMP1B in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP1B fusion protein Ag6222
Full Name chromatin modifying protein 1B
Calculated molecular weight 22 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC065933
Gene symbol CHMP1B
Gene ID (NCBI) 57132
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHMP1B antibody 14639-1-APDownload protocol
IHC protocol for CHMP1B antibody 14639-1-APDownload protocol
IP protocol for CHMP1B antibody 14639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

MBio

Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.

Authors - Ekansh Mittal
humanIF

Elife (Cambridge)

Structure of cellular ESCRT-III spirals and their relationship to HIV budding.

Authors - Anil G Cashikar
humanIF, WB

Dev Cell

NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.

Authors - Oliver B Davis
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier