CHMP1B Polyclonal antibody

CHMP1B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14639-1-AP

Synonyms

C10orf2, C18 ORF2, C18orf2, CHMP1.5, CHMP1B, chromatin modifying protein 1B, hVps46 2, Vps46 2



Tested Applications

Positive WB detected inA431 cells, human heart tissue, mouse heart tissue, rat heart tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14639-1-AP targets CHMP1B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP1B fusion protein Ag6222
Full Name chromatin modifying protein 1B
Calculated molecular weight 22 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC065933
Gene symbol CHMP1B
Gene ID (NCBI) 57132
RRIDAB_2079353
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHMP1B antibody 14639-1-APDownload protocol
IHC protocol for CHMP1B antibody 14639-1-APDownload protocol
IP protocol for CHMP1B antibody 14639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
humanWB

J Extracell Vesicles

Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation

Authors - Pauline P Marie
humanIF, WB

Dev Cell

NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.

Authors - Oliver B Davis
humanIF

Elife (Cambridge)

Structure of cellular ESCRT-III spirals and their relationship to HIV budding.

Authors - Anil G Cashikar
human,mouseIF

MBio

Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.

Authors - Ekansh Mittal
humanIF

Life Sci Alliance

Vaccinia virus hijacks ESCRT-mediated multivesicular body formation for virus egress.

Authors - Moona Huttunen