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- KD/KO Validated
CHMP2A Polyclonal antibody
CHMP2A Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10477-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 13 publications below |
| IF | See 15 publications below |
Product Information
10477-1-AP targets CHMP2A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHMP2A fusion protein Ag0719 |
| Full Name | chromatin modifying protein 2A |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC002502 |
| Gene symbol | CHMP2A |
| Gene ID (NCBI) | 27243 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHMP2A antibody 10477-1-AP | Download protocol |
| IHC protocol for CHMP2A antibody 10477-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Methods Mol Biol Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT. | ||
Dev Cell CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope. | ||
PLoS Genet AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody. | ||
Mol Biol Cell MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis. | ||
Sci Rep VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Stephen (Verified Customer) (04-08-2019) | IF image of Methanol fixed a dividing HeLa cell Stained with CHMP2A antibody (green) and Tubulin Antibody (red)
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