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CHMP2A Polyclonal antibody

CHMP2A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10477-1-AP

Synonyms

CHMP2, CHMP2A, chromatin modifying protein 2A, hVps2 1, VPS2, Vps2 1, VPS2A



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10477-1-AP targets CHMP2A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP2A fusion protein Ag0719
Full Name chromatin modifying protein 2A
Calculated molecular weight 25 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC002502
Gene symbol CHMP2A
Gene ID (NCBI) 27243
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).

Protocols

Product Specific Protocols
WB protocol for CHMP2A antibody 10477-1-APDownload protocol
IHC protocol for CHMP2A antibody 10477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Methods Mol Biol

Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.

Authors - Shai Adar-Levor
humanIF

Dev Cell

CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.

Authors - Leandro N Ventimiglia
humanIF

PLoS Genet

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.

Authors - Chiara Merigliano
humanIF

Mol Biol Cell

MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis.

Authors - Lee Seongju S
humanWB

J Virol

A non-envelopment role for the ESCRT-III complex during HCMV infection.

Authors - Nicholas T Streck
mouseIF

Sci Rep

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Authors - Carolyn Ott

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Stephen (Verified Customer) (04-08-2019)

IF image of Methanol fixed a dividing HeLa cell Stained with CHMP2A antibody (green) and Tubulin Antibody (red)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: HeLa
CHMP2A Antibody Immunofluorescence, validation (1:300 dilution) in HeLa (Cat no:10477-1-AP)