|Positive WB detected in||HepG2 cells, HEK-293 cells, HeLa cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10477-1-AP targets CHMP2A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHMP2A fusion protein Ag0719|
|Full Name||chromatin modifying protein 2A|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC002502|
|Gene ID (NCBI)||27243|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes
ESCRT-III controls nuclear envelope reformation.
Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.
Nat Cell Biol
Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.
Nat Cell Biol
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Stephen (Verified Customer) (04-08-2019)
IF image of Methanol fixed a dividing HeLa cell Stained with CHMP2A antibody (green) and Tubulin Antibody (red)