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WB analysis using 12048-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12048-1-AP (CHN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHN1 Polyclonal antibody

Name: CHN1 Polyclonal antibody
Brand: Proteintech
SKU: 12048-1-AP

CHN1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12048-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

A chimaerin, Alpha chimerin, ARHGAP2, chimerin (chimaerin) 1, CHN, CHN1, DURS2, N chimaerin, N chimerin, NC, RHOGAP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, PC-12 cells
Positive IHC detected in	human gliomas tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 2 publications below

Product Information

12048-1-AP targets CHN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2678 Product name: Recombinant human CHN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC011393 Sequence: MALTLFDTDEYRPPVWKSYLYQLQQEAPHPRRITCTCEVENRPKYYGREFHGMISREAADQLLIVAEGSYLIRESQRQPGTYTLALRFGSQTRNFRLYYDGKHFVGEKRFESIHDLVTDGLITLYIETKAAEYIAKMTINPIYEHVGYTTLNREPAYKKHMPVLKETHDERDSTGQDGVSEKRLTSLVRRATLKENEQIPKYEKIHNFKVHTFRGPHWCEYCANFMWGLIAQGVKCADCGLNVHKQCSKMVPNDCKPDLKHVKKVYSCDLTTLVKAHTTKRPMVVDMCIREIESRGLNSEGLYRVSGFSDLIEDVKMAFDRDGEKADISVNMYEDINIITGALKLYFRDL Predict reactive species
Full Name	chimerin (chimaerin) 1
Calculated Molecular Weight	459 aa, 53 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC011393
Gene Symbol	CHN1
Gene ID (NCBI)	1123
RRID	AB_10794446
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15882
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CHN1 antibody 12048-1-AP	Download protocol
WB protocol for CHN1 antibody 12048-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	BMC Cancer Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis. Authors - Jianbing Liu View Article
human	WB,IHC	Bioengineered Bioinformatics-based analysis of the association between the A1-chimaerin (CHN1) gene and gastric cancer Authors - Jie-Pin Li View Article