CHOP; GADD153 Monoclonal antibody
CHOP; GADD153 Monoclonal Antibody for WB,ELISA
Host / Isotype
Mouse / IgG2a
human and More (2)
WB, IHC, IF, ELISA
Cat no : 60304-1-Ig
|Positive WB detected in||Tunicamycin treated HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
|WB||See 8 publications below|
|IHC||See 1 publications below|
|IF||See 1 publications below|
60304-1-Ig targets CHOP; GADD153 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CHOP; GADD153 fusion protein Ag7354|
|Full Name||DNA-damage-inducible transcript 3|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC003637|
|Gene ID (NCBI)||1649|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).
|Product Specific Protocols|
|WB protocol for CHOP; GADD153 antibody 60304-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.
Cell Death Dis
YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma.
Hum Mol Genet
The role of the ER stress-response protein PERK in rhodopsin retinitis pigmentosa.
Deptor transcriptionally regulates endoplasmic reticulum homeostasis in multiple myeloma cells.
Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation.
ATF6-mediated unfolded protein response facilitates AAV2 transduction by releasing the suppression of AAV receptor on ER stress