CHOP; GADD153 Monoclonal antibody

CHOP; GADD153 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4D5A9

Cat no : 60304-1-Ig

Synonyms

CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153



Tested Applications

Positive WB detected inTunicamycin treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60304-1-Ig targets CHOP; GADD153 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CHOP; GADD153 fusion protein Ag7354
Full Name DNA-damage-inducible transcript 3
Calculated molecular weight 19 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003637
Gene symbol DDIT3
Gene ID (NCBI) 1649
RRIDAB_2881418
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Protocols

Product Specific Protocols
WB protocol for CHOP; GADD153 antibody 60304-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.

Authors - Tengfei Huang
humanWB,IHC

Cell Death Dis

YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma.

Authors - Adrian Rivera-Reyes
ratWB

Hum Mol Genet

The role of the ER stress-response protein PERK in rhodopsin retinitis pigmentosa.

Authors - Dimitra Athanasiou
human, mouseWB

Oncotarget

Deptor transcriptionally regulates endoplasmic reticulum homeostasis in multiple myeloma cells.

Authors - Valeria Catena
humanWB

Apoptosis

Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation.

Authors - Xiaoli Liu
humanWB

J Virol

ATF6-mediated unfolded protein response facilitates AAV2 transduction by releasing the suppression of AAV receptor on ER stress

Authors - Mengtian Cui