CHOP; GADD153 Monoclonal antibody

CHOP; GADD153 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

4D5A9

Cat no : 60304-1-Ig

Synonyms

CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153

---

Validation Data Gallery

• 

  WB analysis of HeLa using 60304-1-Ig

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60304-1-Ig (CHOP; GADD153 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Tunicamycin treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

60304-1-Ig targets CHOP; GADD153 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHOP; GADD153 fusion protein Ag7354
• Full Name: DNA-damage-inducible transcript 3
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC003637
• Gene Symbol: CHOP
• Gene ID (NCBI): 1649
• RRID: AB_2881418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Publications

Species	Application	Title
human	WB	Autophagy; Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.; Authors - Tengfei Huang
human	WB,IHC	Cell Death Dis; YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma.; Authors - Adrian Rivera-Reyes
rat	WB	Hum Mol Genet; The role of the ER stress-response protein PERK in rhodopsin retinitis pigmentosa.; Authors - Dimitra Athanasiou
human, mouse	WB	Oncotarget; Deptor transcriptionally regulates endoplasmic reticulum homeostasis in multiple myeloma cells.; Authors - Valeria Catena
human	WB	Apoptosis; Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation.; Authors - Xiaoli Liu
human	WB	J Virol; ATF6-mediated unfolded protein response facilitates AAV2 transduction by releasing the suppression of AAV receptor on ER stress; Authors - Mengtian Cui