|Positive WB detected in||Tunicamycin treated HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
60304-1-Ig targets CHOP; GADD153 in WB, IHC applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CHOP; GADD153 fusion protein Ag7354|
|Full Name||DNA-damage-inducible transcript 3|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC003637|
|Gene ID (NCBI)||1649|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).
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