CHOP; GADD153 Monoclonal antibody

CHOP; GADD153 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4F3G1

Cat no : 66741-1-Ig

Synonyms

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP; GADD153, CHOP10, DDIT 3, DDIT3, GADD153



Tested Applications

Positive WB detected inHSC-T6 cells, HepG2 cells, C6 cells, NIH/3T3 cells, Tunicamycin treated HepG2 cells
Positive IHC detected inhuman cervical cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66741-1-Ig targets CHOP; GADD153 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, shrew
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CHOP; GADD153 fusion protein Ag7354
Full Name DNA-damage-inducible transcript 3
Calculated molecular weight 19 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003637
Gene symbol DDIT3
Gene ID (NCBI) 1649
RRIDAB_2882089
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Protocols

Product Specific Protocols
WB protocol for CHOP; GADD153 antibody 66741-1-IgDownload protocol
IHC protocol for CHOP; GADD153 antibody 66741-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genome Biol

Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes

Authors - Iliana Soto
human,mouseWB,IF

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu
mouseWB

Front Immunol

miR-10a-3p modulates adiposity and suppresses adipose inflammation through TGF-β1/Smad3 signaling pathway

Authors - Sonia Kiran
humanWB

Biochem Pharmacol

2-Deoxy-D-glucose increases the sensitivity of glioblastoma cells to BCNU through the regulation of glycolysis, ROS and ERS pathways: In vitro and in vivo validation.

Authors - Xiaodong Sun
humanWB

Ecotoxicol Environ Saf

The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin.

Authors - Jing Wu
ratWB

Ecotoxicol Environ Saf

Role of endoplasmic reticulum stress in cadmium-induced hepatocyte apoptosis and the protective effect of quercetin.

Authors - Jicang Wang