Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10613-1-AP targets CHRNA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0933 Product name: Recombinant human CHRNA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-269 aa of BC006314 Sequence: MEPWPLLLLFSLCSAGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGGCGCHDCCC Predict reactive species |
| Full Name | cholinergic receptor, nicotinic, alpha 1 (muscle) |
| Calculated Molecular Weight | 54.5 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC006314 |
| Gene Symbol | CHRNA1 |
| Gene ID (NCBI) | 1134 |
| RRID | AB_2877737 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02708 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CHRNA1 antibody 10613-1-AP | Download protocol |
| WB protocol for CHRNA1 antibody 10613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHRNA1 Polyclonal antibody (Cat# 10613-1-AP) has 16 citations published across 14 journals including Autophagy, Cell Mol Life Sci, Transl Psychiatry, Sci Rep, Aging (Albany NY), Biomaterials Research, Molecular Therapy Nucleic Acids, Biomolecules, Frontiers in Pharmacology, Clinical and Experimental Immunology, Journal of Neuropathology & Experimental Neurology, BMC Musculoskeletal Disorders, Gene, and Biochemical and Biophysical Research Communications. Research fields span neuromuscular junction biology, skeletal muscle, acetylcholine receptor function, gut-brain axis, osteoarthritis, ALS, myasthenia gravis, and lung adenocarcinoma.
| Species | Application | Title |
|---|---|---|
Autophagy E-cigarette aerosols induce the hydrolysis of lysosomal glycerophospholipids through PLA2G4A activation initiated by nicotine binding to CHRNA3/α3 nAchr in airway epithelial cells. | ||
Biomater Res Self-curling 3D oriented scaffolds from fish scales for skeletal muscle regeneration | ||
Transl Psychiatry A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota. | ||
Cell Mol Life Sci The miR-206-3p/Cpeb1 axis delays acetylcholine receptor degradation and preserves neuromuscular junction stability in denervation-induced muscle atrophy. | ||
Mol Ther Nucleic Acids MiR-142a-3p: A novel ACh receptor transcriptional regulator in association with peripheral nerve injury | ||
Aging (Albany NY) Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis. |
